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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0672

PubMed

34757131, Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30.

Characterization method

Smith degradation,NMR,mass spectrometry

Genomic accession number

MZ064058

Nucelotide position range

3333-25157

Substrate

capsule polysaccharide

Loci

None

Species

Acinetobacter baumannii Ab-46-1632/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - QWY12736.1 0 - 2184 (-) MZ064058.1:3333-5517 -
wzb - QWY12737.1 2202 - 2631 (-) MZ064058.1:5535-5964 -
wza - QWY12738.1 2635 - 3736 (-) MZ064058.1:5968-7069 -
gna - QWY12739.1 4091 - 5366 (+) MZ064058.1:7424-8699 -
gtr78 - QWY12740.1 5395 - 6259 (+) MZ064058.1:8728-9592 -
wzx - QWY12741.1 6258 - 7695 (+) MZ064058.1:9591-11028 -
ugd4 - QWY12742.1 7691 - 8855 (+) MZ064058.1:11024-12188 -
rmlB - QWY12743.1 8873 - 9941 (+) MZ064058.1:12206-13274 -
rmlD - QWY12744.1 9943 - 10837 (+) MZ064058.1:13276-14170 -
rmlA - QWY12745.1 10833 - 11724 (+) MZ064058.1:14166-15057 -
rmlC - QWY12746.1 11713 - 12268 (+) MZ064058.1:15046-15601 -
gtr221 - QWY12747.1 12287 - 13103 (+) MZ064058.1:15620-16436 -
wzy - QWY12748.1 13120 - 14086 (+) MZ064058.1:16453-17419 -
gtr81 - QWY12749.1 14106 - 14997 (+) MZ064058.1:17439-18330 -
gtr82 - QWY12750.1 14989 - 15793 (+) MZ064058.1:18322-19126 -
itrA3 - QWY12751.1 15829 - 16432 (+) MZ064058.1:19162-19765 -
galU - QWY12752.1 16466 - 17342 (+) MZ064058.1:19799-20675 -
ugd - QWY12753.1 17365 - 18628 (+) MZ064058.1:20698-21961 -
gpi - QWY12754.1 18624 - 20301 (+) MZ064058.1:21957-23634 -
pgm - QWY12755.1 20454 - 21825 (-) MZ064058.1:23787-25158 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2184 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 2203 - 2631 (-) other Yes
- 2636 - 3736 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 4092 - 5366 (+) other Yes
- 5396 - 6259 (+) CAZyme: GT2 Yes
- 6259 - 7695 (+) other Yes
- 7692 - 8855 (+) other Yes
- 8874 - 9941 (+) other Yes
- 9944 - 10837 (+) other Yes
- 10834 - 11724 (+) other Yes
- 11714 - 12268 (+) other Yes
- 12288 - 13103 (+) CAZyme: GT2|GT2 Yes
- 13121 - 14086 (+) other Yes
- 14107 - 14997 (+) CAZyme: GT2|GT2 Yes
- 14990 - 15793 (+) CAZyme: GT2 Yes
- 15830 - 16432 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 16467 - 17342 (+) CDS No
- 17366 - 18628 (+) CDS No
- 18625 - 20301 (+) CDS No
- 20455 - 21825 (-) CDS No

PUL ID

PUL0672

PubMed

34757131, Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30.

Title

Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis.

Author

Kenyon JJ, Kasimova AA, Sviridova AN, Shpirt AM, Shneider MM, Mikhaylova YV, Shelenkov AA, Popova AV, Perepelov AV, Shashkov AS, Dmitrenok AS, Chizov AO, Knirel YA

Abstract

Whole genome sequence from Acinetobacter baumannii isolate Ab-46-1632 reveals a novel KL144 capsular polysaccharide (CPS) biosynthesis gene cluster, which carries genes for d-glucuronic acid (D-GlcA) and l-rhamnose (l-Rha) synthesis. The CPS was extracted from Ab-46-1632 and studied by (1)H and (13)C NMR spectroscopy, including a two-dimensional (1)H,(13)C HMBC experiment and Smith degradation. The CPS was found to have a hexasaccharide repeat unit composed of four l-Rhap residues and one residue each of d-GlcpA and N-acetyl-d-glucosamine (D-GlcpNAc) consistent with sugar synthesis genes present in KL144. The K144 CPS structure was established and found to be related to those of A. baumannii K55, K74, K85, and K86. A comparison of the corresponding gene clusters to KL144 revealed a number of shared glycosyltransferase genes correlating to shared glycosidic linkages in the structures. One from the enzymes, encoded by only KL144 and KL86, is proposed to be a novel multifunctional rhamnosyltransfaerase likely responsible for synthesis of a shared alpha-l-Rhap-(1 --> 2)-alpha-L-Rhap-(1 --> 3)-L-Rhap trisaccharide fragment in the K144 and K86 structures.