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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0457

PubMed

27142164, Microb Cell Fact. 2016 May 3;15:72. doi: 10.1186/s12934-016-0473-z.

Characterization method

high performance anion exchange chromatography,enzyme activity assay,RNA-Seq

Genomic accession number

NZ_AKZK01000039.1

Nucelotide position range

17308-27154

Substrate

xylooligosaccharide

Loci

A33G_RS0103395-A33G_RS0103425

Species

Lactobacillus rossiae/231049

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- A33G_RS0103395 WP_017261046.1 17308 - 17869 (+) NZ_AKZK01000039.1:34616-35177 -
- A33G_RS0103400 WP_017261047.1 17974 - 19030 (-) NZ_AKZK01000039.1:35282-36338 -
- A33G_RS0103405 WP_017261048.1 19123 - 20752 (-) NZ_AKZK01000039.1:36431-38060 -
- A33G_RS0103410 WP_017261049.1 20798 - 22259 (-) NZ_AKZK01000039.1:38106-39567 -
- A33G_RS0103415 WP_017261050.1 22393 - 23788 (-) NZ_AKZK01000039.1:39701-41096 -
xylA A33G_RS0103420 WP_017261051.1 24176 - 25517 (+) NZ_AKZK01000039.1:41484-42825 5.3.1.5
xylB A33G_RS0103425 WP_017261052.1 25651 - 27154 (+) NZ_AKZK01000039.1:42959-44462 2.7.1.17

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 17309 - 17869 (+) CDS No
- 17975 - 19030 (-) CDS No
- 19124 - 20752 (-) CAZyme: GH43_11|GH43 Yes
- 20799 - 22259 (-) TC: gnl|TC-DB|P94488|2.A.2.3.2 Yes
- 22394 - 23788 (-) TC: gnl|TC-DB|O52733|2.A.1.1.41 Yes
- 24177 - 25517 (+) CDS No
- 25652 - 27154 (+) CDS No

PUL ID

PUL0457

PubMed

27142164, Microb Cell Fact. 2016 May 3;15:72. doi: 10.1186/s12934-016-0473-z.

Title

Cloning, expression and characterization of a beta-D-xylosidase from Lactobacillus rossiae DSM 15814(T).

Author

Pontonio E, Mahony J, Di Cagno R, O'Connell Motherway M, Lugli GA, O'Callaghan A, De Angelis M, Ventura M, Gobbetti M, van Sinderen D

Abstract

BACKGROUND: Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties. Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities. Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes. Endo-xylanases and beta-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan. Xylanase cocktails are used on an industrial scale for biotechnological purposes. Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability. In this study, the capacity of this strain to utilize XOS, xylan, D-xylose and L-arabinose was investigated. RESULTS: Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively. The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) beta-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43. Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 degrees C. Furthermore, beta-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions. CONCLUSIONS: This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.