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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0555

PubMed

25139987, Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.

Characterization method

gene deletion mutant and growth assay,qRT-PCR,microarray,enzyme activity assay

Genomic accession number

NC_016776.1

Nucelotide position range

437949-454517

Substrate

N-glycan

Loci

BF638R_0384-BF638R_0392

Species

Bacteroides fragilis/817

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BF638R_RS01790 WP_008658119.1 0 - 1221 (-) NC_016776.1:437949-439170 -
- BF638R_RS01795 WP_008658120.1 1220 - 3308 (-) NC_016776.1:439169-441257 -
- BF638R_RS01800 WP_008658121.1 3364 - 4900 (-) NC_016776.1:441313-442849 -
- BF638R_RS01805 WP_005784150.1 4930 - 5842 (-) NC_016776.1:442879-443791 -
- BF638R_RS01810 WP_008658122.1 5862 - 7074 (-) NC_016776.1:443811-445023 -
- BF638R_RS01815 WP_008658123.1 7077 - 8985 (-) NC_016776.1:445026-446934 -
- BF638R_RS01820 WP_008658124.1 9336 - 11184 (-) NC_016776.1:447285-449133 -
- BF638R_RS01825 WP_014298189.1 11201 - 14555 (-) NC_016776.1:449150-452504 -
- BF638R_RS01830 WP_008658127.1 14919 - 16569 (+) NC_016776.1:452868-454518 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1221 (-) CAZyme: GH154 Yes
- 1221 - 3308 (-) CAZyme: GH2 Yes
- 3365 - 4900 (-) CAZyme: GH20 Yes
- 4931 - 5842 (-) other Yes
- 5863 - 7074 (-) CAZyme: GH88 Yes
- 7078 - 8985 (-) CAZyme: PL0|PL33_1 Yes
- 9337 - 11184 (-) other Yes
- 11202 - 14555 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 14920 - 16569 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No

PUL ID

PUL0555

PubMed

25139987, Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.

Title

Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections.

Author

Cao Y, Rocha ER, Smith CJ

Abstract

Bacteroides fragilis is the most common anaerobe isolated from clinical infections, and in this report we demonstrate a characteristic of the species that is critical to their success as an opportunistic pathogen. Among the Bacteroides spp. in the gut, B. fragilis has the unique ability of efficiently harvesting complex N-linked glycans from the glycoproteins common to serum and serous fluid. This activity is mediated by an outer membrane protein complex designated as Don. Using the abundant serum glycoprotein transferrin as a model, it has been shown that B. fragilis alone can rapidly and efficiently deglycosylate this protein in vitro and that transferrin glycans can provide the sole source of carbon and energy for growth in defined media. We then showed that transferrin deglycosylation occurs in vivo when B. fragilis is propagated in the rat tissue cage model of extraintestinal growth, and that this ability provides a competitive advantage in vivo over strains lacking the don locus.