Database for Polyphenol Utilized Proteins from gut microbiota
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Subfamily

Family Sequences


Literature Information

Title Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents
Author Anne Bertrand, Sandrine Morel, François Lefoulon, Yves Rolland, Pierre Monsan, Magali Remaud-Simeon
DOI 10.1016/j.carres.2006.02.008
Abstract The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3’-O-alpha-d-glucopyranoside and luteolin-4’-O-alpha-d-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively.

Experimental results

  • Enzyme

Uniprot ID: Q9RE05

Protein: Dextransucrase

Organism: Leuconostoc mesenteroides

Length: 2,057 AA

Taxonomic identifier: 1245 [NCBI]

  • Pfam
Source Domain Start End E-value (Domain) Coverage
Pfam-A Glyco_hydro_70 453 1312 4.8e-302 0.998

Program: hmmscan

Version: 3.1b2 (February 2015)

Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa

Date: Mon Jul 20 14:32:16 2020

Description:

Glyco_hydro_70

Pfam

Members of this family belong to glycosyl hydrolase family 70[1] Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) catalyse the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules[2] EC:2.4.1.5. This family roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the Putative cell wall binding domain PF01473 which corresponds with the C-terminal glucan-binding domain.

InterPro

O-Glycosyl hydrolases (3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [3][4]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) (2.4.1.5, GH70) catalyse the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules [2:1]. This entry represents a domain that roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the putative cell wall binding domain PF01473 which corresponds with the C-terminal glucan-binding domain.

Information is taken from Pfam and InterPro web site.

  • Reaction

luteolinluteolin-3’-O-α-D-glucopyranoside + luteolin-4’-O-α-Dglucopyranoside + luteolin-4’-O-α-D-tri-glucoside + luteolin-4’-O-α-di-glucosides

References

  1. Henrissat B. A classification of glycosyl hydrolases based on amino acid sequence similarities[J]. Biochemical journal, 1991, 280(2): 309-316. ↩︎

  2. Monchois V, Willemot R M, Remaud-Simeon M, et al. Cloning and sequencing of a gene coding for a novel dextransucrase from Leuconostoc mesenteroides NRRL B-1299 synthesizing only α (1–6) and α (1–3) linkages[J]. Gene, 1996, 182(1-2): 23-32. ↩︎ ↩︎

  3. Henrissat B, Callebaut I, Fabrega S, et al. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases[J]. Proceedings of the National Academy of Sciences, 1995, 92(15): 7090-7094. ↩︎

  4. Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases[J]. Structure, 1995, 3(9): 853-859. ↩︎

Literature Information

Title Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents
Author Anne Bertrand, Sandrine Morel, François Lefoulon, Yves Rolland, Pierre Monsan, Magali Remaud-Simeon
DOI 10.1016/j.carres.2006.02.008
Abstract The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3’-O-alpha-d-glucopyranoside and luteolin-4’-O-alpha-d-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively.

Experimental results

  • Enzyme

Uniprot ID: Q9ZAR4

Protein: Dextransucrase

Organism: Leuconostoc mesenteroides

Length: 1,527 AA

Taxonomic identifier: 1245 [NCBI]

  • Pfam
Source Domain Start End E-value (Domain) Coverage
Pfam-A Glyco_hydro_70 370 1169 0 0.999

Program: hmmscan

Version: 3.1b2 (February 2015)

Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa

Date: Mon Jul 20 14:32:16 2020

Description:

Glyco_hydro_70

Pfam

Members of this family belong to glycosyl hydrolase family 70[1] Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) catalyse the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules[2] EC:2.4.1.5. This family roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the Putative cell wall binding domain PF01473 which corresponds with the C-terminal glucan-binding domain.

InterPro

O-Glycosyl hydrolases (3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [3][4]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) (2.4.1.5, GH70) catalyse the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules [2:1]. This entry represents a domain that roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the putative cell wall binding domain PF01473 which corresponds with the C-terminal glucan-binding domain.

Information is taken from Pfam and InterPro web site.

  • Reaction

luteolinluteolin-3’-O-α-D-glucopyranoside + luteolin-4’-O-α-Dglucopyranoside

[L1]luteolin
right_arrow
[R1]luteolin-3'-O-a-D-glucopyranoside
plus
[R2]luteolin-4'-O-a-D-glucopyranoside

References

  1. Henrissat B. A classification of glycosyl hydrolases based on amino acid sequence similarities[J]. Biochemical journal, 1991, 280(2): 309-316. ↩︎

  2. Monchois V, Willemot R M, Remaud-Simeon M, et al. Cloning and sequencing of a gene coding for a novel dextransucrase from Leuconostoc mesenteroides NRRL B-1299 synthesizing only α (1–6) and α (1–3) linkages[J]. Gene, 1996, 182(1-2): 23-32. ↩︎ ↩︎

  3. Henrissat B, Callebaut I, Fabrega S, et al. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases[J]. Proceedings of the National Academy of Sciences, 1995, 92(15): 7090-7094. ↩︎

  4. Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases[J]. Structure, 1995, 3(9): 853-859. ↩︎


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