Database for Polyphenol Utilized Proteins from gut microbiota
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Family Sequences


Literature Information

Title Identification of a Novel Dihydrodaidzein Racemase Essential for Biosynthesis of Equol from Daidzein in Lactococcus sp. Strain 20-92
Author Yoshikazu Shimada, Masayuki Takahashi, Norihiro Miyazawa, Yasuhiro Abiru, Shigeto Uchiyama, Haretsugu Hishigaki
DOI 10.1128/AEM.00410-12
Abstract Equol is metabolized from daidzein, a soyisoflavone, by the gut microflora. In this study, we identified a novel dihydrodaidzein racemase (l-DDRC) that is involved in equol biosynthesis in a lactic acid bacterium, Lactococcus sp. strain 20-92, and confirmed that histidine-tagged recombinant l-DDRC (l-DDRC-His) was able to convert both the (R)- and (S)-enantiomers of dihydrodaidzein to the racemate. Moreover, we showed that recombinant l-DDRC-His was essential for in vitro equol production from daidzein by a recombinant enzyme mixture and that efficient in vitro equol production from daidzein was possible using at least four enzymes, including l-DDRC. We also proposed a model of the metabolic pathway from daidzein to equol in Lactococcus strain 20-92.

Experimental results

  • Enzyme

Uniprot ID: I7H868

Protein: Dihydrodaizein racemase

Organism: Lactococcus garvieae

Length: 158 AA

Taxonomic identifier: 1363 [NCBI]

  • Pfam
Source Domain Start End E-value (Domain) Coverage
Pfam-A Glyoxalase_4 10 104 6.5e-11 0.844

Program: hmmscan

Version: 3.1b2 (February 2015)

Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa

Date: Mon Jul 20 14:32:16 2020

Description:

Glyoxalase_4

Pfam

No Pfam abstract.

InterPro

No InterPro results.

Information is taken from Pfam and InterPro web site.

  • Reaction

(R)-dihydrodaidzein(S)-dihydrodaidzein

[L1](R)-dihydrodaidzein
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[R1](S)-dihydrodaidzein

References


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