Database for Polyphenol Utilized Proteins from gut microbiota
Loading…
View sequence similarity network

Subfamily

Family Sequences


Literature Information

Title First bacterial chalcone isomerase isolated from Eubacterium ramulus
Author Claudia Herles , Annett Braune, Michael Blaut
DOI 10.1007/s00203-004-0676-2
Abstract The human fecal anaerobe Eubacterium ramulus is capable of degrading various flavonoids, including the flavone naringenin. The first step in the proposed degradation pathway is the isomerization of naringenin to the corresponding chalcone. Cell-free extracts of E. ramulus displayed chalcone isomerase activity. The enzyme from E. ramulus was purified to homogeneity. Its apparent molecular mass was estimated to be 136 and 129 kDa according to gel filtration and native polyacrylamide gel electrophoresis, respectively. Chalcone isomerase is composed of one type of subunit of 30 kDa. The purified enzyme catalyzed the isomerization of naringenin chalcone, isoliquiritigenin, and butein, three chalcones that differ in their hydroxylation pattern. N-bromosuccinimide, but also naringenin and phloretin, inhibited the purified enzyme considerably. This is the first report on a bacterial chalcone isomerase. The physiological function of the purified enzyme is unclear, but an involvement in the conversion of the flavanone naringenin to the chalcone is proposed.

Experimental results

  • Enzyme

Uniprot ID: V9P0A9

Protein: Chalcone isomerase

Organism: Eubacterium ramulus

Length: 283 AA

Taxonomic identifier: 39490 [NCBI]

  • Pfam
Source Domain Start End E-value (Domain) Coverage
Pfam-A Chalcone_N 6 106 2.8e-49 0.980

Program: hmmscan

Version: 3.1b2 (February 2015)

Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa

Date: Mon Jul 20 14:32:16 2020

Description:

Chalcone_N

Pfam

This is the N-terminal domain of chalcone isomerase present in Eubacterium ramulus. Chalcone isomerase is involved in the degradation pathway of flavone naringenin1.

InterPro

This is the N-terminal domain of chalcone isomerase present in Eubacterium ramulus. Chalcone isomerase is involved in the degradation pathway of flavone naringenin1.

  • Reaction

naringenin chalcone3-(4-hydroxyphenyl)propionic acid + phloroglucinol

[L1]naringenin chalcone
right_arrow
[R1]3-(4-hydroxyphenyl)propionic acid
plus
[R2]phloroglucinol

References


  1. Herles C, Braune A, Blaut M. First bacterial chalcone isomerase isolated from Eubacterium ramulus[J]. Archives of microbiology, 2004, 181(6): 428-434. 


Copyright © 2023 University of Nebraska-Lincoln | Dr. Yin's Lab at UNL
Handcoded by Pengxiang Zhang & Yinchao He.