| Title | Characterization and application of an acidophilic and thermostable β-glucosidase from Thermofilum pendens |
|---|---|
| Author | Dan Li, Xiaolei Li, Wei Dang, Phuong Lan Tran, Sung-Hoon Park, Byung-Chul Oh, Wan-Soo Hong, Jin-Sil Lee, Kwan-Hwa Park |
| DOI | 10.1016/j.jbiosc.2012.11.009 |
| Abstract | The gene encoding a β-glucosidase from the archaeon Thermofilum pendens (Tpbgl) was cloned and expressed in Escherichia coli. The purified recombinant enzyme had a molecular mass of 77.8 kDa and released glucose or mannose from p-nitrophenyl-β-d-glucopyranoside (pNPG), cellobiose, mannobiose, and genistin. Peak Tpbgl activity was detected at 90°C, and 50% activity remained after incubation for 60 min at 95°C. The optimal pH for pNPG hydrolysis was 3.5. When the enzyme was incubated with pNPG in the presence of ethanol and propanol, the glucose moiety was transferred to acceptor alcohols. Tpbgl is the archaeal β-glucosidase from glucoside hydrolase family 3 and found to be most heat stable under extremely acidic conditions (pH 3.5). The kinetic parameters revealed that Tpbgl had the highest catalytic efficiency toward pNPG (kcat/Km = 3.05) with strong substrate affinity for such natural substrates as cellobiose (Km = 0.149) and mannobiose (Km = 0.147). Genistin solubilized in 10-40% DMSO was hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme. Our results indicate that Tpbgl has great potential in cellulose saccharification and the glucoside hydrolysis of natural compounds. |
Uniprot ID: A1S0B1
Protein: Glycoside hydrolase, family 3 domain protein
Organism: Thermofilum pendens (strain DSM 2475 / Hrk 5)
Length: 701 AA
Taxonomic identifier: 368408 [NCBI]
| Source | Domain | Start | End | E-value (Domain) | Coverage |
|---|---|---|---|---|---|
| Pfam-A | Glyco_hydro_3_C | 312 | 578 | 3.2e-47 | 0.995 |
| Pfam-A | Fn3-like | 611 | 682 | 4.2e-24 | 0.986 |
| Pfam-A | Glyco_hydro_3 | 32 | 279 | 1.3e-59 | 0.811 |
Program: hmmscan
Version: 3.1b2 (February 2015)
Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa
Date: Mon Jul 20 14:32:16 2020
Description:
Glyco_hydro_3_C
This domain is involved in catalysis and may be involved in binding beta-glucan1. This domain is found associated with PF00933.
Glycoside hydrolase family 3 GH3 comprises enzymes with a number of known activities; beta-glucosidase (3.2.1.21); beta-xylosidase (3.2.1.37); N-acetyl beta-glucosaminidase (3.2.1.52); glucan beta-1,3-glucosidase (3.2.1.58); cellodextrinase(3.2.1.74); exo-1,3-1,4-glucanase (3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites1. This entry represents the C-terminal domain, involved in catalysis and may be involved in binding beta-glucan1. It is found associated with PF00933.
Fn3-like
This domain has a fibronectin type III-like structure2. It is often found in association with PF00933 and PF01915. Its function is unknown.
This domain has a fibronectin type III-like structure2. It is often found in association with PF00933 and PF01915. Its function is unknown.
Glyco_hydro_3
No Pfam abstract.
O-Glycosyl hydrolases (3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families34. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.
Glycoside hydrolase family 3 GH3 comprises enzymes with a number of known activities; beta-glucosidase (3.2.1.21); beta-xylosidase (3.2.1.37); N-acetyl beta-glucosaminidase (3.2.1.52); glucan beta-1,3-glucosidase (3.2.1.58); cellodextrinase (3.2.1.74); exo-1,3-1,4-glucanase (3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites1. This domain is often N-terminal to the glycoside hydrolase family 3, C-terminal domain IPR002772.
Information is taken from Pfam and InterPro web site.
p-nitrophenyl-β-D-glucopyranoside ⇒ p-nitrophenyl + β-D-Glucopyranose
![[L1]p-nitrophenyl-β-D-glucopyranoside](../static/images/chemical_structure/HR3/HR3_2/[L1]p-nitrophenyl-β-D-glucopyranoside.png)
![[R1]p-nitrophenyl](../static/images/chemical_structure/HR3/HR3_2/[R1]p-nitrophenyl.png)
![[R2]β-D-Glucopyranose](../static/images/chemical_structure/HR3/HR3_2/[R2]β-D-Glucopyranose.png)
Varghese J N, Hrmova M, Fincher G B. Three-dimensional structure of a barley β-D-glucan exohydrolase, a family 3 glycosyl hydrolase[J]. Structure, 1999, 7(2): 179-190. ↩↩↩↩
Pozzo T, Pasten J L, Karlsson E N, et al. Structural and functional analyses of β-glucosidase 3B from Thermotoga neapolitana: a thermostable three-domain representative of glycoside hydrolase 3[J]. Journal of molecular biology, 2010, 397(3): 724-739. ↩↩
Henrissat B, Callebaut I, Fabrega S, et al. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases[J]. Proceedings of the National Academy of Sciences, 1995, 92(15): 7090-7094. ↩
Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases[J]. Structure, 1995, 3(9): 853-859. ↩