| Title | Characterization of four β-glucosidases acting on isoflavone-glycosides from Bifidobacterium pseudocatenulatum IPLA 36007 |
|---|---|
| Author | Lucía Guadamuro, Ana Belén Flórez, Ángel Alegría, Lucía Vázquez, Baltasar Mayo |
| DOI | 10.1016/j.foodres.2017.07.024 |
| Abstract | Bifidobacterium pseudocatenulatum IPLA 36007 acts on isoflavone glycosides, releasing their corresponding aglycones. This strain-specific activity might be a key step in making isoflavones bioavailable and harnessing their oestrogenic activity. To investigate the molecular mechanisms involved in this activity, four glycosyl hydrolase-encoding genes in the IPLA 36007 genome (AW18_01575, AW18_09810, AW18_08145, and AW18_08090) were selected, synthesized with heterologous promoter and terminator signals (r-β-gluA, r-β-gluB, r-β-gluD and r-β-gluE, respectively), cloned into Escherichia coli, overexpressed as His-tagged proteins, and the enzymes purified and characterized. All four enzymes – GluAHis, GluBHis, GluDHis and GluEHis – proved to have β-glucosidase activity and deglycosylated (although at different rates) the isoflavone glycosides daidzin and genistin, releasing the aglycone moieties daidzein and genistein, respectively. GluDHis and GluEHis were also shown to hydrolyse β-glucosyl disaccharides such as cellobiose and gentiobiose, while GluAHis and GluBHis did not. Differences in activity were recorded for all four β-glucosidases at different pHs and temperatures under otherwise similar assay conditions, suggesting they have complementary activities under different environmental conditions. Two of the recombinant genes, r-β-gluA, and r-β-gluD, were cloned and expressed in the model lactic acid bacterium Lactococcus lactis, suggesting starter and probiotic organisms could be endowed with β-glucosidase activity. B. pseudocatenulatum IPLA 36007 contains additional β-glucosidases to those studied in this work, indicating a high level of redundancy for this enzymatic activity. Knowledge of glycoside-degrading enzymes should facilitate the development of novel, more effective or more selective prebiotics or functional foods for the promotion of bifidobacterial numbers in the human gut. It might also be of interest in the development of novel probiotics with specific health-promoting activities. |
Uniprot ID: A0A072MRT2
Protein: Beta-D-glucosideglucohydrolase
Organism: Bifidobacterium pseudocatenulatum IPLA36007
Length: 780 AA
Taxonomic identifier: 1454376 [NCBI]
| Source | Domain | Start | End | E-value (Domain) | Coverage |
|---|---|---|---|---|---|
| Pfam-A | Glyco_hydro_3_C | 383 | 675 | 2.7e-42 | 0.990 |
| Pfam-A | Fn3-like | 698 | 767 | 4.8e-20 | 0.986 |
| Pfam-A | Glyco_hydro_3 | 32 | 342 | 2.4e-67 | 0.994 |
Program: hmmscan
Version: 3.1b2 (February 2015)
Method: hmmscan --domtblout hmmscan.tbl --noali -E 1e-5 pfam query.fa
Date: Mon Jul 20 14:32:16 2020
Description:
Glyco_hydro_3_C
This domain is involved in catalysis and may be involved in binding beta-glucan1. This domain is found associated with PF00933.
Glycoside hydrolase family 3 GH3 comprises enzymes with a number of known activities; beta-glucosidase (3.2.1.21); beta-xylosidase (3.2.1.37); N-acetyl beta-glucosaminidase (3.2.1.52); glucan beta-1,3-glucosidase (3.2.1.58); cellodextrinase(3.2.1.74); exo-1,3-1,4-glucanase (3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites1. This entry represents the C-terminal domain, involved in catalysis and may be involved in binding beta-glucan1. It is found associated with PF00933.
Fn3-like
This domain has a fibronectin type III-like structure2. It is often found in association with PF00933 and PF01915. Its function is unknown.
This domain has a fibronectin type III-like structure2. It is often found in association with PF00933 and PF01915. Its function is unknown.
Glyco_hydro_3
No Pfam abstract.
O-Glycosyl hydrolases (3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families34. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.
Glycoside hydrolase family 3 GH3 comprises enzymes with a number of known activities; beta-glucosidase (3.2.1.21); beta-xylosidase (3.2.1.37); N-acetyl beta-glucosaminidase (3.2.1.52); glucan beta-1,3-glucosidase (3.2.1.58); cellodextrinase (3.2.1.74); exo-1,3-1,4-glucanase (3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites1. This domain is often N-terminal to the glycoside hydrolase family 3, C-terminal domain IPR002772.
Information is taken from Pfam and InterPro web site.
p-nitrophenyl-β-D-glucopyranoside ⇒ p-nitrophenyl + β-D-Glucopyranose
![[L1]p-nitrophenyl-β-D-glucopyranoside](../static/images/chemical_structure/HR3/HR3_3/[L1]p-nitrophenyl-β-D-glucopyranoside.png)
![[R1]p-nitrophenyl](../static/images/chemical_structure/HR3/HR3_3/[R1]p-nitrophenyl.png)
![[R2]β-D-Glucopyranose](../static/images/chemical_structure/HR3/HR3_3/[R2]β-D-Glucopyranose.png)
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Henrissat B, Callebaut I, Fabrega S, et al. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases[J]. Proceedings of the National Academy of Sciences, 1995, 92(15): 7090-7094. ↩
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