APIS family ID | APIS002 | |||||||||||||
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Inhibited defense system | cyclic oligonucleotide-based antiphage signaling system (CBASS) | |||||||||||||
CLAN ID | CLAN018 | |||||||||||||
Seed protein property |
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Phage property |
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PMID/References | PMID:36848932 | |||||||||||||
PDB structures | 7UQ2; | |||||||||||||
Pfam domains | ||||||||||||||
Phrog | phrog_717,phrog_26803 | |||||||||||||
Host taxa | d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli | |||||||||||||
Gene Location | Start: 63078; End: 63344; Strand: - | |||||||||||||
Description | Vs.4 antagonized cGAS signaling by binding tightly to cGAMP (dissociation constant of approximately 30 nM) and sequestering it; Bioinformatic analyses identified 198 homologues of Vs.4 in diverse phages; belongs to PHROG 717. |
Genome accession | Start | End | Strand | Protein ID | Length | Molecular weight | Charge | Isoelectric point | Function | Phrog | PFAM |
---|---|---|---|---|---|---|---|---|---|---|---|
NC_000866.4 | 60922 | 61389 | - | NP_049723.1 | 155 | 17489.28 | -0.5 | 6.2245 | phosphatase | phrog_15329 ; phrog_335 | PF01661.24 |
NC_000866.4 | 61386 | 61733 | - | NP_049724.1 | 115 | 13055.82 | 5.5 | 8.8579 | valyl tRNA synthetase modifier | phrog_1256 ; phrog_38498 | |
NC_000866.4 | 61726 | 62271 | - | NP_049725.1 | 181 | 20680.73 | 16.0 | 10.2749 | hypothetical protein | phrog_600 | PF01464.23 |
NC_000866.4 | 62279 | 62740 | - | NP_049726.1 | 153 | 17976.58 | 4.5 | 8.5065 | endoribonuclease | phrog_1323 | PF10715.12 |
NC_000866.4 | 62800 | 63078 | - | NP_049727.1 | 92 | 10904.26 | -1.5 | 5.1972 | hypothetical protein | phrog_2037 | |
NC_000866.4 | 63078 | 63344 | - | NP_049728.1 | 88 | 10210.61 | -6.5 | 4.3805 | hypothetical protein | phrog_26803 ; phrog_717 | |
NC_000866.4 | 63381 | 63557 | - | NP_049729.1 | 58 | 6611.00 | 2.0 | 10.3162 | hypothetical protein | phrog_1986 | |
NC_000866.4 | 63557 | 63919 | - | NP_049730.1 | 120 | 13813.65 | -1.5 | 5.9896 | autonomous glycyl radical cofactor GrcA | phrog_21327 ; phrog_1209 ; phrog_32552 | PF01228.24 |
NC_000866.4 | 63927 | 64256 | - | NP_049731.1 | 109 | 12835.07 | 4.5 | 9.3432 | hypothetical protein | phrog_29719 ; phrog_2049 ; phrog_11888 | |
NC_000866.4 | 64253 | 64912 | - | NP_049732.1 | 219 | 25026.71 | 12.5 | 9.4437 | hypothetical protein | phrog_1488 | |
NC_000866.4 | 64939 | 65355 | - | NP_049733.1 | 138 | 16078.57 | 7.0 | 9.7612 | endonuclease V N-glycosylase UV repair enzyme | phrog_1183 ; phrog_18345 | PF03013.17 |
Hit | Query start | Query end | Hit start | Hit end | TMscore(ali) | TMscore(avg) |
---|---|---|---|---|---|---|
MGYP000570486689.pdb | 12 | 83 | 14 | 85 | 0.8888 | 0.7169 |
Fam ID | Seed protein | Member count | Model | Alignment | APIS002 | NP_049728.1 | 103 | HMM model | Member alignment |
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Hit APIS family | E-Value | Query Start | Query End | Hit Start | Hit End | Immune system | Seed protein |
---|---|---|---|---|---|---|---|
APIS004 | 2.41E-06 | 11 | 98 | 25 | 116 | cyclic oligonucleotide-based antiphage signaling system (CBASS) | ANA48877.1 |
Member | pident | evalue | bitscore | qcovs | phage | host | SourceDB | Pfam | Phrog |
---|---|---|---|---|---|---|---|---|---|
AP014714_00126 | 65.934 | 1.22e-30 | 112 | 98% | Edwardsiella phage PEi20 | Edwardsiella | INPHARED | phrog_717 phrog_26803 | |
DQ904452_00113 | 93.182 | 1.28e-53 | 169 | 99% | Escherichia phage RB32 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
HE956711_00119 | 94.318 | 9.41e-54 | 170 | 99% | Yersinia phage phiD1 | Yersinia | INPHARED | phrog_717 phrog_26803 | |
IMGVR_UViG_2974743505_000001|2974743505|2974743738 | 94.382 | 5.09e-56 | 176 | 100% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_2974775753_000001|2974775753|2974775864 | 98.876 | 8.58e-59 | 183 | 100% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_3300045988_081160|3300045988|Ga0495776_047593_92644_92907 | 71.591 | 3.59e-35 | 123 | 99% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_3300045988_095701|3300045988|Ga0495776_073022_68358_68624 | 90.909 | 7.44e-52 | 165 | 99% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_3300045988_118042|3300045988|Ga0495776_119731_85662_85928 | 67.033 | 5.94e-32 | 115 | 98% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_3300045988_136812|3300045988|Ga0495776_009634_64378_64647 | 78.161 | 1.13e-43 | 144 | 98% | IMGVR | phrog_717 phrog_26803 | |||
IMGVR_UViG_3300045988_154884|3300045988|Ga0495776_101933_84990_85253 | 67.045 | 1.47e-31 | 114 | 99% | IMGVR | phrog_717 phrog_26803 | |||
JX128259_00115 | 98.864 | 3.10e-58 | 181 | 99% | Escherichia phage ECML-134 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
JX561091_00078 | 77.011 | 2.06e-43 | 144 | 98% | Escherichia phage phAPEC8 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
KF208315_00113 | 98.864 | 6.12e-58 | 181 | 99% | Yersinia phage PST | Yersinia | INPHARED | phrog_717 phrog_26803 | |
KJ477686_00114 | 98.864 | 7.21e-58 | 180 | 99% | Tequatrovirus T4 | INPHARED | phrog_717 phrog_26803 | ||
KM607000_00115 | 93.182 | 7.64e-54 | 170 | 99% | Enterobacteria phage RB27 | Enterobacteria | INPHARED | phrog_717 phrog_26803 | |
KU867876_00115 | 97.727 | 6.27e-57 | 178 | 99% | Escherichia phage vB_EcoM-UFV13 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
KX130861_00025 | 97.727 | 4.19e-56 | 176 | 99% | Shigella phage SHFML-11 | Shigella | INPHARED | phrog_717 phrog_26803 | |
KX130862_00217 | 93.182 | 7.64e-54 | 170 | 99% | Shigella phage SHFML-26 | Shigella | INPHARED | phrog_717 phrog_26803 | |
KX664695_00063 | 75.862 | 3.03e-42 | 141 | 98% | Escherichia phage ESCO5 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
LR215722_00114 | 94.318 | 6.72e-53 | 168 | 99% | Yersinia phage fPS-2 | Yersinia | INPHARED | phrog_717 phrog_26803 | |
LR215724_00119 | 94.318 | 1.99e-55 | 174 | 99% | Yersinia phage fPS-65 | Yersinia | INPHARED | phrog_717 phrog_26803 | |
LR597654_00083 | 78.161 | 8.10e-44 | 145 | 98% | Escherichia phage phAPEC8_ev052 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
LR597657_00110 | 95.455 | 3.10e-54 | 171 | 99% | Escherichia phage T4_ev240 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MF327007_00112 | 94.318 | 1.47e-54 | 172 | 99% | Shigella phage Sf21 | Shigella | INPHARED | phrog_717 phrog_26803 | |
MF327008_00242 | 97.727 | 4.68e-56 | 176 | 99% | Shigella phage Sf24 | Shigella | INPHARED | phrog_717 phrog_26803 | |
MG746602_00027 | 63.218 | 3.36e-31 | 113 | 98% | Klebsiella phage vB_Kpn_F48 | Klebsiella | INPHARED | phrog_717 phrog_26803 | |
MGV-GENOME-0373836_133 | 90.909 | 7.67e-52 | 165 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0375157_138 | 63.218 | 4.45e-31 | 113 | 98% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0375647_162 | 67.045 | 1.51e-31 | 114 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0375924_99 | 78.161 | 1.12e-43 | 144 | 98% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377341_264 | 94.318 | 3.65e-54 | 171 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377451_2 | 96.591 | 1.38e-56 | 177 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377569_131 | 98.864 | 1.26e-57 | 180 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377588_2 | 97.727 | 8.83e-56 | 175 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377591_2 | 100.000 | 1.86e-58 | 182 | 99% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-0377830_131 | 67.033 | 6.11e-32 | 115 | 98% | MGV | phrog_717 phrog_26803 | |||
MGV-GENOME-4435766_177 | 71.591 | 3.70e-35 | 123 | 99% | MGV | phrog_717 phrog_26803 | |||
MH791409_00209 | 66.667 | 3.45e-32 | 115 | 99% | Escherichia phage EcWhh-1 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MH837626_00228 | 95.455 | 6.03e-56 | 175 | 99% | Escherichia phage vB_vPM_PD112 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MH992510_00116 | 89.773 | 1.70e-50 | 162 | 99% | Escherichia phage vB_EcoM_DalCa | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MK373781_00117 | 94.318 | 1.70e-53 | 169 | 99% | Escherichia phage vB_EcoM_KAW1E185 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MK962750_00112 | 94.318 | 4.32e-54 | 171 | 99% | Shigella phage CM8 | Shigella | INPHARED | phrog_717 phrog_26803 | |
MN087708_00121 | 66.667 | 9.31e-31 | 112 | 98% | Enterobacter phage vB_EhoM-IME523 | Enterobacter | INPHARED | phrog_717 phrog_26803 | |
MN716856_00199 | 96.591 | 3.19e-56 | 176 | 99% | Yersinia phage vB_YepM_ZN18 | Yersinia | INPHARED | phrog_717 phrog_26803 | |
MN895434_00131 | 100.000 | 1.51e-57 | 179 | 98% | Escherichia phage teqhad | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MN895437_00192 | 79.518 | 4.60e-37 | 128 | 93% | Escherichia phage teqskov | Escherichia | INPHARED | phrog_717 | |
MN994497_00163 | 94.318 | 2.72e-54 | 171 | 99% | Phage NBEco003 | INPHARED | phrog_717 phrog_26803 | ||
MT129652_00098 | 65.591 | 1.01e-31 | 114 | 99% | Escherichia phage MN01 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MT176424_00117 | 94.318 | 4.87e-54 | 171 | 99% | Citrobacter phage PhiZZ6 | Citrobacter | INPHARED | phrog_717 phrog_26803 | |
MT682713_00117 | 93.182 | 1.28e-53 | 169 | 99% | Escherichia phage vB_EcoM_Ozark | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MT701588_00178 | 66.667 | 2.16e-31 | 114 | 98% | Klebsiella phage Metamorpho | Klebsiella | INPHARED | phrog_717 phrog_26803 | |
MT764206_00231 | 97.727 | 4.72e-57 | 178 | 99% | Escherichia phage JEP6 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MT782071_00114 | 68.966 | 9.71e-31 | 112 | 98% | Escherichia phage JN02 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MW175890_00207 | 78.161 | 1.09e-43 | 144 | 98% | Dompiswa phage TSP7_1 | Dompiswa | INPHARED | phrog_717 phrog_26803 | |
MW250787_00184 | 65.591 | 2.11e-31 | 114 | 99% | Escherichia phage UPEC07 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MW286157_00221 | 97.727 | 3.83e-57 | 178 | 99% | Escherichia phage vB_EcoM-BECP11 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MW598459_00144 | 65.591 | 4.59e-32 | 115 | 99% | Escherichia phage vB_EcoM_RZ | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MW679410_00115 | 65.591 | 2.65e-31 | 113 | 99% | Escherichia phage C6 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MZ170040_00079 | 92.045 | 7.92e-53 | 167 | 99% | Escherichia phage vB_EcoM_FJ1 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MZ417521_00112 | 97.727 | 3.79e-57 | 178 | 99% | Escherichia phage 132 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MZ501098_00225 | 93.182 | 1.56e-53 | 169 | 99% | Escherichia phage PaulHMueller | Escherichia | INPHARED | phrog_717 phrog_26803 | |
MZ571829_00076 | 63.218 | 4.32e-31 | 113 | 98% | Klebsiella phage vB_KpnM-VAC36 | Klebsiella | INPHARED | phrog_717 phrog_26803 | |
MZ726795_00117 | 93.182 | 5.70e-53 | 168 | 99% | Escherichia phage vB_EcoM_ASO78A | Escherichia | INPHARED | phrog_717 phrog_26803 | |
NP_049728.1 | 100.000 | 5.62e-65 | 181 | 100% | T4 of E. coli | Escherichia coli | NCBI | phrog_717 phrog_26803 | |
OK040907_00116 | 68.817 | 2.29e-32 | 116 | 99% | Enterobacteria phage Ac3 | Enterobacteria | INPHARED | phrog_717 phrog_26803 | |
OK331996_00186 | 96.591 | 2.60e-55 | 174 | 99% | Escherichia phage vB_EcoM-G157lw | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OL656107_00117 | 90.909 | 5.72e-52 | 165 | 99% | Salmonella phage UAB_60 | Salmonella | INPHARED | phrog_717 phrog_26803 | |
OL770073_00139 | 95.455 | 1.52e-55 | 174 | 99% | Escherichia phage vB_EcoM-RPN226 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OL770107_00054 | 66.667 | 4.59e-32 | 115 | 99% | Escherichia phage CLB_P2 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OL960580_00116 | 94.318 | 3.74e-54 | 171 | 99% | Escherichia phage vB_EcoM_SA21RB | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OL960581_00115 | 98.864 | 7.21e-58 | 180 | 99% | Escherichia phage vB_EcoM_SA20RB | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OM339528_00153 | 100.000 | 4.04e-52 | 166 | 90% | Enterobacteria phage SV76 | Enterobacteria | INPHARED | phrog_717 phrog_26803 | |
OM386655_00093 | 65.909 | 1.83e-30 | 111 | 99% | Escherichia phage vB_EcoM_ESCO10 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OM386662_00051 | 67.045 | 1.18e-30 | 112 | 99% | Escherichia phage vB_EcoM_ESCO47 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OM386666_00145 | 92.045 | 4.11e-52 | 166 | 99% | Escherichia phage vB_EcoM_ESCO58 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
ON210139_00160 | 97.727 | 2.92e-56 | 176 | 99% | Escherichia phage EC128 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
ON528735_00105 | 96.000 | 1.19e-44 | 147 | 84% | Shigella phage ESh25 | Shigella | INPHARED | phrog_717 phrog_26803 | |
ON528740_00181 | 98.864 | 6.39e-58 | 180 | 99% | Shigella phage ESh30 | Shigella | INPHARED | phrog_717 phrog_26803 | |
ON528741_00135 | 93.182 | 2.05e-53 | 169 | 99% | Shigella phage ESh31 | Shigella | INPHARED | phrog_717 phrog_26803 | |
ON528742_00006 | 95.455 | 7.13e-55 | 172 | 99% | Shigella phage ESh32 | Shigella | INPHARED | phrog_717 phrog_26803 | |
ON645936_00114 | 94.318 | 4.22e-54 | 171 | 99% | Escherichia phage N2 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
ON782582_00238 | 94.318 | 7.31e-54 | 170 | 99% | Escherichia phage ECP52 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP137232_00257 | 94.318 | 4.98e-54 | 171 | 99% | Shigella phage S2_01 | Shigella | INPHARED | phrog_717 phrog_26803 | |
OP168898_00149 | 98.864 | 2.44e-57 | 179 | 99% | Escherichia phage ZCEC14 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP172784_00212 | 98.864 | 1.63e-57 | 179 | 99% | Escherichia phage ECO07P1 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP292222_00126 | 65.591 | 3.69e-31 | 113 | 99% | Escherichia phage Esco_phi_B-01 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP555982_00116 | 94.318 | 3.54e-54 | 171 | 99% | Escherichia phage vB_EcoM-UFV11 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP611477_00227 | 93.182 | 1.99e-53 | 169 | 99% | Escherichia virus CAM-21 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP870144_00113 | 95.455 | 4.63e-56 | 176 | 99% | Escherichia phage vB_Eco_F25 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OP870145_00118 | 98.864 | 7.05e-58 | 180 | 99% | Escherichia phage vB_Eco_F26 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ031492_00059 | 77.011 | 1.39e-42 | 142 | 98% | Escherichia phage MRostami-2023b | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ067373_00225 | 93.182 | 1.48e-53 | 169 | 99% | Escherichia phage a15 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ067375_00223 | 100.000 | 1.81e-58 | 182 | 99% | Escherichia phage a48 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ067376_00220 | 95.455 | 7.13e-55 | 172 | 99% | Escherichia phage a51 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ174502_00232 | 90.909 | 1.84e-52 | 167 | 99% | Escherichia phage REP3 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ446694_00141 | 96.591 | 1.34e-56 | 177 | 99% | Escherichia phage Killian | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ466432_00004 | 95.455 | 1.12e-54 | 172 | 99% | Escherichia phage ADUt | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ599309_00117 | 97.727 | 8.56e-56 | 175 | 99% | Escherichia phage 107 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ599310_00274 | 70.115 | 4.60e-31 | 112 | 98% | Escherichia phage 55 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ721911_00114 | 98.864 | 6.14e-57 | 178 | 99% | Escherichia phage vB_VIPECOOM03 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
OQ721912_00122 | 67.033 | 1.13e-31 | 114 | 98% | Enterobacter phage vB_VIPECLOM01 | Enterobacter | INPHARED | phrog_717 phrog_26803 | |
OV877511_00204 | 68.966 | 2.05e-30 | 111 | 98% | Escherichia phage UGJNEcP2 | Escherichia | INPHARED | phrog_717 phrog_26803 | |
uvig_352869_22 | 98.864 | 1.22e-57 | 180 | 99% | GPD | phrog_717 phrog_26803 |
APIS | Contig | Range |
---|---|---|
APIS002 | NC_000866.4 | 60922 - 65355 |