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Seed proteins  | Seed genomic context  | Seed protein 3D structures  |  Structural homologs  |  Family infomation  | Homology to other families  | Family members, sources, and their hosts  

Seed protein information help

APIS family ID APIS002
Inhibited defense system cyclic oligonucleotide-based antiphage signaling system (CBASS)
CLAN ID CLAN018
Seed protein property
Accession ID Gene name Protein length Molecular weight Isoelectric point Charge
NP_049728.1 gene_page: vs.4 88 10210.6 4.3805 -6.5
Phage property
Phage Assembly id
T4 of E. coli GCF_000836945.1
PMID/References PMID:36848932
PDB structures 7UQ2;
Pfam domains
Phrog phrog_717,phrog_26803
Host taxa d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli
Gene Location Start: 63078;  End: 63344;  Strand: -
Description Vs.4 antagonized cGAS signaling by binding tightly to cGAMP (dissociation constant of approximately 30 nM) and sequestering it; Bioinformatic analyses identified 198 homologues of Vs.4 in diverse phages; belongs to PHROG 717.

Seed genomic context      Download table help

Genome accession Start End Strand Protein ID Length Molecular weight Charge Isoelectric point Function Phrog PFAM
NC_000866.4 60922 61389 - NP_049723.1 155 17489.28 -0.5 6.2245 phosphatase phrog_15329 ; phrog_335 PF01661.24
NC_000866.4 61386 61733 - NP_049724.1 115 13055.82 5.5 8.8579 valyl tRNA synthetase modifier phrog_1256 ; phrog_38498
NC_000866.4 61726 62271 - NP_049725.1 181 20680.73 16.0 10.2749 hypothetical protein phrog_600 PF01464.23
NC_000866.4 62279 62740 - NP_049726.1 153 17976.58 4.5 8.5065 endoribonuclease phrog_1323 PF10715.12
NC_000866.4 62800 63078 - NP_049727.1 92 10904.26 -1.5 5.1972 hypothetical protein phrog_2037
NC_000866.4 63078 63344 - NP_049728.1 88 10210.61 -6.5 4.3805 hypothetical protein phrog_26803 ; phrog_717
NC_000866.4 63381 63557 - NP_049729.1 58 6611.00 2.0 10.3162 hypothetical protein phrog_1986
NC_000866.4 63557 63919 - NP_049730.1 120 13813.65 -1.5 5.9896 autonomous glycyl radical cofactor GrcA phrog_21327 ; phrog_1209 ; phrog_32552 PF01228.24
NC_000866.4 63927 64256 - NP_049731.1 109 12835.07 4.5 9.3432 hypothetical protein phrog_29719 ; phrog_2049 ; phrog_11888
NC_000866.4 64253 64912 - NP_049732.1 219 25026.71 12.5 9.4437 hypothetical protein phrog_1488
NC_000866.4 64939 65355 - NP_049733.1 138 16078.57 7.0 9.7612 endonuclease V N-glycosylase UV repair enzyme phrog_1183 ; phrog_18345 PF03013.17

Seed genomic context- JBrowse     

PDB structure: 7UQ2 ; Download help

Full Sequence      Download help

Structural homologs

No homologs found in AlphaFold database

Structure homologs against by esmdb (html) ; download full result table

Hit Query start Query end Hit start Hit end TMscore(ali) TMscore(avg)
MGYP000570486689.pdb 12 83 14 85 0.8888 0.7169

Family information      Download help

Fam ID Seed protein Member count Model Alignment
APIS002 NP_049728.1 103 HMM model Member alignment

Host Taxa distribution

Length distribution

Homology to other families       download full data without filtering help

Clan ID: CLAN018

Hit APIS family E-Value Query Start Query End Hit Start Hit End Immune system Seed protein
APIS004 2.41E-06 11 98 25 116 cyclic oligonucleotide-based antiphage signaling system (CBASS) ANA48877.1

Family members, sources, and their hosts        help

Results of representative protein blast against members.
Member pident evalue bitscore qcovs phage host SourceDB Pfam Phrog
AP014714_00126 65.934 1.22e-30 112 98% Edwardsiella phage PEi20 Edwardsiella INPHARED phrog_717 phrog_26803
DQ904452_00113 93.182 1.28e-53 169 99% Escherichia phage RB32 Escherichia INPHARED phrog_717 phrog_26803
HE956711_00119 94.318 9.41e-54 170 99% Yersinia phage phiD1 Yersinia INPHARED phrog_717 phrog_26803
IMGVR_UViG_2974743505_000001|2974743505|2974743738 94.382 5.09e-56 176 100% IMGVR phrog_717 phrog_26803
IMGVR_UViG_2974775753_000001|2974775753|2974775864 98.876 8.58e-59 183 100% IMGVR phrog_717 phrog_26803
IMGVR_UViG_3300045988_081160|3300045988|Ga0495776_047593_92644_92907 71.591 3.59e-35 123 99% IMGVR phrog_717 phrog_26803
IMGVR_UViG_3300045988_095701|3300045988|Ga0495776_073022_68358_68624 90.909 7.44e-52 165 99% IMGVR phrog_717 phrog_26803
IMGVR_UViG_3300045988_118042|3300045988|Ga0495776_119731_85662_85928 67.033 5.94e-32 115 98% IMGVR phrog_717 phrog_26803
IMGVR_UViG_3300045988_136812|3300045988|Ga0495776_009634_64378_64647 78.161 1.13e-43 144 98% IMGVR phrog_717 phrog_26803
IMGVR_UViG_3300045988_154884|3300045988|Ga0495776_101933_84990_85253 67.045 1.47e-31 114 99% IMGVR phrog_717 phrog_26803
JX128259_00115 98.864 3.10e-58 181 99% Escherichia phage ECML-134 Escherichia INPHARED phrog_717 phrog_26803
JX561091_00078 77.011 2.06e-43 144 98% Escherichia phage phAPEC8 Escherichia INPHARED phrog_717 phrog_26803
KF208315_00113 98.864 6.12e-58 181 99% Yersinia phage PST Yersinia INPHARED phrog_717 phrog_26803
KJ477686_00114 98.864 7.21e-58 180 99% Tequatrovirus T4 INPHARED phrog_717 phrog_26803
KM607000_00115 93.182 7.64e-54 170 99% Enterobacteria phage RB27 Enterobacteria INPHARED phrog_717 phrog_26803
KU867876_00115 97.727 6.27e-57 178 99% Escherichia phage vB_EcoM-UFV13 Escherichia INPHARED phrog_717 phrog_26803
KX130861_00025 97.727 4.19e-56 176 99% Shigella phage SHFML-11 Shigella INPHARED phrog_717 phrog_26803
KX130862_00217 93.182 7.64e-54 170 99% Shigella phage SHFML-26 Shigella INPHARED phrog_717 phrog_26803
KX664695_00063 75.862 3.03e-42 141 98% Escherichia phage ESCO5 Escherichia INPHARED phrog_717 phrog_26803
LR215722_00114 94.318 6.72e-53 168 99% Yersinia phage fPS-2 Yersinia INPHARED phrog_717 phrog_26803
LR215724_00119 94.318 1.99e-55 174 99% Yersinia phage fPS-65 Yersinia INPHARED phrog_717 phrog_26803
LR597654_00083 78.161 8.10e-44 145 98% Escherichia phage phAPEC8_ev052 Escherichia INPHARED phrog_717 phrog_26803
LR597657_00110 95.455 3.10e-54 171 99% Escherichia phage T4_ev240 Escherichia INPHARED phrog_717 phrog_26803
MF327007_00112 94.318 1.47e-54 172 99% Shigella phage Sf21 Shigella INPHARED phrog_717 phrog_26803
MF327008_00242 97.727 4.68e-56 176 99% Shigella phage Sf24 Shigella INPHARED phrog_717 phrog_26803
MG746602_00027 63.218 3.36e-31 113 98% Klebsiella phage vB_Kpn_F48 Klebsiella INPHARED phrog_717 phrog_26803
MGV-GENOME-0373836_133 90.909 7.67e-52 165 99% MGV phrog_717 phrog_26803
MGV-GENOME-0375157_138 63.218 4.45e-31 113 98% MGV phrog_717 phrog_26803
MGV-GENOME-0375647_162 67.045 1.51e-31 114 99% MGV phrog_717 phrog_26803
MGV-GENOME-0375924_99 78.161 1.12e-43 144 98% MGV phrog_717 phrog_26803
MGV-GENOME-0377341_264 94.318 3.65e-54 171 99% MGV phrog_717 phrog_26803
MGV-GENOME-0377451_2 96.591 1.38e-56 177 99% MGV phrog_717 phrog_26803
MGV-GENOME-0377569_131 98.864 1.26e-57 180 99% MGV phrog_717 phrog_26803
MGV-GENOME-0377588_2 97.727 8.83e-56 175 99% MGV phrog_717 phrog_26803
MGV-GENOME-0377591_2 100.000 1.86e-58 182 99% MGV phrog_717 phrog_26803
MGV-GENOME-0377830_131 67.033 6.11e-32 115 98% MGV phrog_717 phrog_26803
MGV-GENOME-4435766_177 71.591 3.70e-35 123 99% MGV phrog_717 phrog_26803
MH791409_00209 66.667 3.45e-32 115 99% Escherichia phage EcWhh-1 Escherichia INPHARED phrog_717 phrog_26803
MH837626_00228 95.455 6.03e-56 175 99% Escherichia phage vB_vPM_PD112 Escherichia INPHARED phrog_717 phrog_26803
MH992510_00116 89.773 1.70e-50 162 99% Escherichia phage vB_EcoM_DalCa Escherichia INPHARED phrog_717 phrog_26803
MK373781_00117 94.318 1.70e-53 169 99% Escherichia phage vB_EcoM_KAW1E185 Escherichia INPHARED phrog_717 phrog_26803
MK962750_00112 94.318 4.32e-54 171 99% Shigella phage CM8 Shigella INPHARED phrog_717 phrog_26803
MN087708_00121 66.667 9.31e-31 112 98% Enterobacter phage vB_EhoM-IME523 Enterobacter INPHARED phrog_717 phrog_26803
MN716856_00199 96.591 3.19e-56 176 99% Yersinia phage vB_YepM_ZN18 Yersinia INPHARED phrog_717 phrog_26803
MN895434_00131 100.000 1.51e-57 179 98% Escherichia phage teqhad Escherichia INPHARED phrog_717 phrog_26803
MN895437_00192 79.518 4.60e-37 128 93% Escherichia phage teqskov Escherichia INPHARED phrog_717
MN994497_00163 94.318 2.72e-54 171 99% Phage NBEco003 INPHARED phrog_717 phrog_26803
MT129652_00098 65.591 1.01e-31 114 99% Escherichia phage MN01 Escherichia INPHARED phrog_717 phrog_26803
MT176424_00117 94.318 4.87e-54 171 99% Citrobacter phage PhiZZ6 Citrobacter INPHARED phrog_717 phrog_26803
MT682713_00117 93.182 1.28e-53 169 99% Escherichia phage vB_EcoM_Ozark Escherichia INPHARED phrog_717 phrog_26803
MT701588_00178 66.667 2.16e-31 114 98% Klebsiella phage Metamorpho Klebsiella INPHARED phrog_717 phrog_26803
MT764206_00231 97.727 4.72e-57 178 99% Escherichia phage JEP6 Escherichia INPHARED phrog_717 phrog_26803
MT782071_00114 68.966 9.71e-31 112 98% Escherichia phage JN02 Escherichia INPHARED phrog_717 phrog_26803
MW175890_00207 78.161 1.09e-43 144 98% Dompiswa phage TSP7_1 Dompiswa INPHARED phrog_717 phrog_26803
MW250787_00184 65.591 2.11e-31 114 99% Escherichia phage UPEC07 Escherichia INPHARED phrog_717 phrog_26803
MW286157_00221 97.727 3.83e-57 178 99% Escherichia phage vB_EcoM-BECP11 Escherichia INPHARED phrog_717 phrog_26803
MW598459_00144 65.591 4.59e-32 115 99% Escherichia phage vB_EcoM_RZ Escherichia INPHARED phrog_717 phrog_26803
MW679410_00115 65.591 2.65e-31 113 99% Escherichia phage C6 Escherichia INPHARED phrog_717 phrog_26803
MZ170040_00079 92.045 7.92e-53 167 99% Escherichia phage vB_EcoM_FJ1 Escherichia INPHARED phrog_717 phrog_26803
MZ417521_00112 97.727 3.79e-57 178 99% Escherichia phage 132 Escherichia INPHARED phrog_717 phrog_26803
MZ501098_00225 93.182 1.56e-53 169 99% Escherichia phage PaulHMueller Escherichia INPHARED phrog_717 phrog_26803
MZ571829_00076 63.218 4.32e-31 113 98% Klebsiella phage vB_KpnM-VAC36 Klebsiella INPHARED phrog_717 phrog_26803
MZ726795_00117 93.182 5.70e-53 168 99% Escherichia phage vB_EcoM_ASO78A Escherichia INPHARED phrog_717 phrog_26803
NP_049728.1 100.000 5.62e-65 181 100% T4 of E. coli Escherichia coli NCBI phrog_717 phrog_26803
OK040907_00116 68.817 2.29e-32 116 99% Enterobacteria phage Ac3 Enterobacteria INPHARED phrog_717 phrog_26803
OK331996_00186 96.591 2.60e-55 174 99% Escherichia phage vB_EcoM-G157lw Escherichia INPHARED phrog_717 phrog_26803
OL656107_00117 90.909 5.72e-52 165 99% Salmonella phage UAB_60 Salmonella INPHARED phrog_717 phrog_26803
OL770073_00139 95.455 1.52e-55 174 99% Escherichia phage vB_EcoM-RPN226 Escherichia INPHARED phrog_717 phrog_26803
OL770107_00054 66.667 4.59e-32 115 99% Escherichia phage CLB_P2 Escherichia INPHARED phrog_717 phrog_26803
OL960580_00116 94.318 3.74e-54 171 99% Escherichia phage vB_EcoM_SA21RB Escherichia INPHARED phrog_717 phrog_26803
OL960581_00115 98.864 7.21e-58 180 99% Escherichia phage vB_EcoM_SA20RB Escherichia INPHARED phrog_717 phrog_26803
OM339528_00153 100.000 4.04e-52 166 90% Enterobacteria phage SV76 Enterobacteria INPHARED phrog_717 phrog_26803
OM386655_00093 65.909 1.83e-30 111 99% Escherichia phage vB_EcoM_ESCO10 Escherichia INPHARED phrog_717 phrog_26803
OM386662_00051 67.045 1.18e-30 112 99% Escherichia phage vB_EcoM_ESCO47 Escherichia INPHARED phrog_717 phrog_26803
OM386666_00145 92.045 4.11e-52 166 99% Escherichia phage vB_EcoM_ESCO58 Escherichia INPHARED phrog_717 phrog_26803
ON210139_00160 97.727 2.92e-56 176 99% Escherichia phage EC128 Escherichia INPHARED phrog_717 phrog_26803
ON528735_00105 96.000 1.19e-44 147 84% Shigella phage ESh25 Shigella INPHARED phrog_717 phrog_26803
ON528740_00181 98.864 6.39e-58 180 99% Shigella phage ESh30 Shigella INPHARED phrog_717 phrog_26803
ON528741_00135 93.182 2.05e-53 169 99% Shigella phage ESh31 Shigella INPHARED phrog_717 phrog_26803
ON528742_00006 95.455 7.13e-55 172 99% Shigella phage ESh32 Shigella INPHARED phrog_717 phrog_26803
ON645936_00114 94.318 4.22e-54 171 99% Escherichia phage N2 Escherichia INPHARED phrog_717 phrog_26803
ON782582_00238 94.318 7.31e-54 170 99% Escherichia phage ECP52 Escherichia INPHARED phrog_717 phrog_26803
OP137232_00257 94.318 4.98e-54 171 99% Shigella phage S2_01 Shigella INPHARED phrog_717 phrog_26803
OP168898_00149 98.864 2.44e-57 179 99% Escherichia phage ZCEC14 Escherichia INPHARED phrog_717 phrog_26803
OP172784_00212 98.864 1.63e-57 179 99% Escherichia phage ECO07P1 Escherichia INPHARED phrog_717 phrog_26803
OP292222_00126 65.591 3.69e-31 113 99% Escherichia phage Esco_phi_B-01 Escherichia INPHARED phrog_717 phrog_26803
OP555982_00116 94.318 3.54e-54 171 99% Escherichia phage vB_EcoM-UFV11 Escherichia INPHARED phrog_717 phrog_26803
OP611477_00227 93.182 1.99e-53 169 99% Escherichia virus CAM-21 Escherichia INPHARED phrog_717 phrog_26803
OP870144_00113 95.455 4.63e-56 176 99% Escherichia phage vB_Eco_F25 Escherichia INPHARED phrog_717 phrog_26803
OP870145_00118 98.864 7.05e-58 180 99% Escherichia phage vB_Eco_F26 Escherichia INPHARED phrog_717 phrog_26803
OQ031492_00059 77.011 1.39e-42 142 98% Escherichia phage MRostami-2023b Escherichia INPHARED phrog_717 phrog_26803
OQ067373_00225 93.182 1.48e-53 169 99% Escherichia phage a15 Escherichia INPHARED phrog_717 phrog_26803
OQ067375_00223 100.000 1.81e-58 182 99% Escherichia phage a48 Escherichia INPHARED phrog_717 phrog_26803
OQ067376_00220 95.455 7.13e-55 172 99% Escherichia phage a51 Escherichia INPHARED phrog_717 phrog_26803
OQ174502_00232 90.909 1.84e-52 167 99% Escherichia phage REP3 Escherichia INPHARED phrog_717 phrog_26803
OQ446694_00141 96.591 1.34e-56 177 99% Escherichia phage Killian Escherichia INPHARED phrog_717 phrog_26803
OQ466432_00004 95.455 1.12e-54 172 99% Escherichia phage ADUt Escherichia INPHARED phrog_717 phrog_26803
OQ599309_00117 97.727 8.56e-56 175 99% Escherichia phage 107 Escherichia INPHARED phrog_717 phrog_26803
OQ599310_00274 70.115 4.60e-31 112 98% Escherichia phage 55 Escherichia INPHARED phrog_717 phrog_26803
OQ721911_00114 98.864 6.14e-57 178 99% Escherichia phage vB_VIPECOOM03 Escherichia INPHARED phrog_717 phrog_26803
OQ721912_00122 67.033 1.13e-31 114 98% Enterobacter phage vB_VIPECLOM01 Enterobacter INPHARED phrog_717 phrog_26803
OV877511_00204 68.966 2.05e-30 111 98% Escherichia phage UGJNEcP2 Escherichia INPHARED phrog_717 phrog_26803
uvig_352869_22 98.864 1.22e-57 180 99% GPD phrog_717 phrog_26803
APIS Contig Range
APIS002 NC_000866.4 60922 - 65355

Gene cluster comparison plot by clinker      Download html