| APIS family ID | APIS012 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Inhibited defense system | toxin-antitoxin (TA) | |||||||||||||
| CLAN ID | CLAN022 | |||||||||||||
| Seed protein property |
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| Phage property |
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| PMID/References | PMID:35924892 | |||||||||||||
| PDB structures | ; | |||||||||||||
| Pfam domains | ||||||||||||||
| Phrog | phrog_8921,phrog_2375,phrog_3272,phrog_12748 | |||||||||||||
| Host taxa | d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli | |||||||||||||
| Gene Location | Start: 20112; End: 20369; Strand: - | |||||||||||||
| Description | Original gene 61.4, experimentally evolved T4 phage to overcome a phage-defensive toxin-antitoxin system (toxIN) in E. coli. Through recombination, T4 rapidly acquires segmental amplifications of a previously uncharacterized gene, now named tifA, encoding an inhibitor of the toxin, ToxN. | |||||||||||||
| Genome accession | Start | End | Strand | Protein ID | Length | Molecular weight | Charge | Isoelectric point | Function | Phrog | PFAM |
|---|---|---|---|---|---|---|---|---|---|---|---|
| NC_000866.4 | 16846 | 17625 | - | NP_049647.1 | 259 | 30416.80 | 9.5 | 9.2073 | Dam family site-specific DNA-(adenine-N6)-methyltransferase | phrog_56 | PF02086.18 |
| NC_000866.4 | 17935 | 18963 | - | NP_049648.1 | 342 | 39777.99 | 15.5 | 9.5801 | DNA primase | phrog_21887 ; phrog_47 | |
| NC_000866.4 | 18966 | 19130 | - | NP_049649.1 | 54 | 5896.64 | -1.0 | 5.0824 | hypothetical protein | phrog_1823 ; phrog_2086 | PF07068.14 |
| NC_000866.4 | 19132 | 19758 | - | NP_049650.1 | 208 | 24331.57 | 0.0 | 6.4890 | hypothetical protein | phrog_8539 ; phrog_4122 ; phrog_3781 | |
| NC_000866.4 | 19758 | 20051 | - | NP_049651.1 | 97 | 10993.66 | -3.0 | 4.6017 | spackle periplasmic | phrog_1586 | |
| NC_000866.4 | 20112 | 20369 | - | NP_049652.1 | 85 | 10187.10 | 8.5 | 10.4193 | hypothetical protein | phrog_3272 ; phrog_8921 ; phrog_2375 ; phrog_12748 | |
| NC_000866.4 | 20371 | 20553 | - | NP_049653.1 | 60 | 7027.25 | -1.0 | 4.9813 | Dmd discriminator of mRNA degradation | phrog_2102 | PF17587.5 |
| NC_000866.4 | 20612 | 22039 | - | NP_049654.1 | 475 | 53601.01 | -6.5 | 5.2884 | AAA family ATPase | phrog_19 | PF03796.18 |
| NC_000866.4 | 22049 | 22393 | - | NP_049655.1 | 114 | 13290.24 | -6.0 | 4.6272 | head vertex assembly chaperone | phrog_999 | PF11113.11 |
| NC_000866.4 | 22386 | 23561 | - | NP_049656.2 | 391 | 43998.29 | -6.5 | 5.0416 | recombinase | phrog_8337 ; phrog_18112 ; phrog_97 ; phrog_31060 | PF00154.24 |
| NC_000866.4 | 23570 | 24235 | - | NP_049657.1 | 221 | 25339.15 | 25.0 | 10.5097 | homing endonuclease | phrog_19446 ; phrog_37840 ; phrog_314 | PF01541.27 ;PF07460.14 ;PF07460.14 |
pTM is for the estimate of the TM-score, which is obtained from a pairwise error prediction. The higher pTM score indicates better model quality.
pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .
| Fam ID | Seed protein | Member count | Model | Alignment | APIS012 | NP_049652.1 | 73 | HMM model | Member alignment |
|---|
| Hit APIS family | E-Value | Query Start | Query End | Hit Start | Hit End | Immune system | Seed protein |
|---|---|---|---|---|---|---|---|
| APIS086 | 2.51E-59 | 1 | 92 | 2 | 93 | ON229909_00228 |
| APIS | Contig | Range |
|---|---|---|
| APIS012 | NC_000866.4 | 16846 - 24235 |