| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0166 | enzyme activity assay, RT-PCR | starch | Bacteroides fragilis | 16788175 Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis: Regulation by carbon source and oxygen. J Bacteriol. 2006 Jul;188(13):4663-72. doi: 10.1128/JB.00125-06. |
2006 Jul | degradation | 5 | 1 | GH13_10, GH13 |
| PUL0174 | RT-PCR, enzyme activity assay, enzymatic product analysis | starch | Kribbella flavida | 27302067 Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1-->6)-alpha-nigerosyl. J Biol Chem. 2016 Aug 5;291(32):16438-47. doi: 10.1074/jbc.M116.727305. Epub 2016 Jun 14. |
2016 Aug 5 | degradation | 3 | 2 | CBM20, GH31, CBM35, GH31 |
| PUL0236 | proteome fractionation, mass spectrometry, target decoy database analysis | alpha-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 13 | 4 | GH13, GH13, GH13_36, GH65, GH13_7 |
| PUL0268 | Northern Blot, promoter assay | starch | Geobacillus kaustophilus | 23793634 Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426. Appl Environ Microbiol. 2013 Sep;79(17):5151-8. doi: 10.1128/AEM.01506-13. Epub 2013 Jun 21. |
2013 Sep | degradation | 5 | 1 | GH13_1, GH13 |
| PUL0361 | enzyme activity assay | starch, maltodextrin | Thermotoga maritima | 10972187 Thermotoga maritima AglA, an extremely thermostable NAD+-, Mn2+-, and thiol-dependent alpha-glucosidase. Extremophiles. 2000 Aug;4(4):189-200. doi: 10.1007/pl00010711. |
2000 Aug | degradation | 6 | 3 | GH13_20, CBM34, GH13, GH4 |
| PUL0421 | microarray | starch | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 7 | 3 | GH13, GH13_10, GH97, GH13 |
| PUL0469 | microarray, qPCR | starch | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 8 | 3 | GH97 |
| PUL0473 | growth assay | alpha-glucan, starch | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 8 | 3 | CBM26, GH13, GH97, GH13 |
| PUL0478 | growth assay | alpha-glucan, starch | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 8 | 4 | GH2, GH92, GH92, GH125 |
| PUL0589 | Western Blot, enzyme activity assay, thin layer chromatography | starch | Streptococcus mutans | 23930155 The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. doi: 10.3402/jom.v5i0.21285. Print 2013. |
2013 | degradation | 3 | 2 | GT35, GH77 |
| PUL0595 | enzyme activity assay, qPCR, crystallization | starch | [Eubacterium] rectale | 25388295 Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19. |
2015 Jan | degradation | 4 | 1 | CBM26, GH13_41, CBM82, GH13, CBM41, CBM83 |
| PUL0596 | enzyme activity assay, qPCR, crystallization | starch | [Eubacterium] rectale | 25388295 Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19. |
2015 Jan | degradation | 4 | 1 | GH13_36 |
| PUL0611 | liquid chromatography and mass spectrometry | alpha-glucan | Winogradskyella sp. isolate Bin3 | 32071270 Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19. |
2020 Feb 18 | degradation | 14 | 6 | GH97, GH13, GH13_19, GH13_38, GH31, GH13, GH65 |
| PUL0612 | liquid chromatography and mass spectrometry | alpha-glucan | Muricauda sp. isolate Bin2 | 32071270 Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19. |
2020 Feb 18 | degradation | 10 | 2 | GH65, GH13_38 |
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