| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000233177 |
| Family | GH16 |
| Protein Properties | Length: 291 Molecular Weight: 32780.8 Isoelectric Point: 8.3146 |
| Chromosome | Chromosome/Scaffold: 012168191 Start: 14093 End: 15547 |
| Description | xyloglucan:xyloglucosyl transferase 33 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 42 | 208 | 1.3e-24 |
| NIQVINGSMATLALDKNSGSGLASVNKFHYGFFSAAIKLPAGLTSGVVVAFYLSNADVFPHNHDEIDIELLGHDKRNDWVIQTNIYANGSVNTGREEKFY LWFDPTLQHHQYSIIWNNHHTVFLVDNVPVREFQHSSSFFPSKPMSVYATIWDASEWATHGGKYPVN | |||
| Full Sequence |
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| Protein Sequence Length: 291 Download |
| MAFFXGKIHF WRYTSPSVPR LTDVFPRVSI DRGFSKVFGG SNIQVINGSM ATLALDKNSG 60 SGLASVNKFH YGFFSAAIKL PAGLTSGVVV AFYLSNADVF PHNHDEIDIE LLGHDKRNDW 120 VIQTNIYANG SVNTGREEKF YLWFDPTLQH HQYSIIWNNH HTVFLVDNVP VREFQHSSSF 180 FPSKPMSVYA TIWDASEWAT HGGKYPVNYK YAPFRVSFAE MEMSGCISNP TGTVTSCARS 240 NPSSVDPVEG PEFVKLSSQQ SGAMDWARSK LMFYSYCKDT KRFKVMPPEC K 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 2.0e-18 | 40 | 205 | 176 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-18 | 41 | 200 | 170 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-58 | 31 | 211 | 182 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 2.0e-59 | 25 | 290 | 276 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 1.0e-118 | 28 | 290 | 268 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABA54988.1 | 0 | 13 | 290 | 24 | 304 | xyloglucan endotransglycosylase hydrolase [Apium graveolens] |
| GenBank | ABM91064.1 | 0 | 12 | 291 | 29 | 312 | xyloglucan endotransglycosylase/hydrolase precursor XTH-38 [Populus tremula x Populus tremuloides] |
| RefSeq | NP_172525.1 | 0 | 9 | 290 | 22 | 309 | XTH33; hydrolase, acting on glycosyl bonds / hydrolase, hydrolyzing O-glycosyl compounds / xyloglucan:xyloglucosyl transferase [Arabidopsis thaliana] |
| RefSeq | XP_002263411.1 | 0 | 14 | 291 | 30 | 311 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002320268.1 | 0 | 12 | 291 | 29 | 312 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2uwc_B | 0 | 28 | 290 | 17 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwc_A | 0 | 28 | 290 | 17 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwa_C | 0 | 28 | 290 | 20 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_B | 0 | 28 | 290 | 20 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_A | 0 | 28 | 290 | 20 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| AM715612 | 269 | 13 | 280 | 0 |
| FF391444 | 246 | 48 | 290 | 0 |
| CX710395 | 245 | 49 | 290 | 0 |
| FS319984 | 235 | 13 | 243 | 0 |
| CX047376 | 253 | 9 | 256 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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