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Seed proteins  | Seed genomic context  | Seed protein 3D structures  |  Structural homologs  |  Family infomation  | Homology to other families  | Family members, sources, and their hosts  

Seed protein information help

APIS family ID APIS005
Inhibited defense system Gabija
CLAN ID CLAN004
Seed protein property
Accession ID Gene name Protein length Molecular weight Isoelectric point Charge
APD21271.1 gene_page: Gad1 295 34865.17 7.0151 2.0
Phage property
Phage Assembly id
phi3T of B. subtilis GCA_002601445.1
PMID/References Yirmiya et al., 2023 Antine et al., 2023
PDB structures ;
Pfam domains
Phrog phrog_20113
Host taxa d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis
Gene Location Start: 87269;  End: 88156;  Strand: +
Description Gad1 forms an octameric web that encases the GajAB complex and inhibits DNA recognition and cleavage.

Seed genomic context      Download table help

Genome accession Start End Strand Protein ID Length Molecular weight Charge Isoelectric point Function Phrog PFAM
KY030782.1 85148 85945 - APD21266.1 265 30604.26 7.0 8.4156 ATP-dependent DNA ligase phrog_114 PF01068.24
KY030782.1 86030 86461 - APD21267.1 143 16619.95 -3.0 5.0112 hypothetical protein phrog_1599 ; phrog_20165 PF02586.17
KY030782.1 86550 86771 + APD21268.1 73 8710.92 -3.0 4.5701 hypothetical protein phrog_5179
KY030782.1 86772 86891 + APD21269.1 39 4506.24 0.0 5.8591 hypothetical protein
KY030782.1 86999 87220 + APD21270.1 73 8954.41 6.0 10.2799 hypothetical protein phrog_19340
KY030782.1 87269 88156 + APD21271.1 295 34865.17 2.0 7.0151 hypothetical protein phrog_20113
KY030782.1 88146 88418 + APD21272.1 90 10952.38 -3.5 4.7672 hypothetical protein phrog_6343
KY030782.1 88421 88567 + APD21273.1 48 5661.33 -6.5 4.1220 hypothetical protein phrog_29081
KY030782.1 88573 88722 + APD21274.1 49 5507.25 -2.5 4.6472 hypothetical protein phrog_25134
KY030782.1 88785 89165 + APD21275.1 126 15019.29 -1.5 5.2358 hypothetical protein phrog_20815
KY030782.1 89449 90075 + APD21276.1 208 23826.84 -13.0 4.4578 hypothetical protein phrog_24226 PF09716.13

Seed genomic context- JBrowse     

Predicted 3D structure by alphafold2 with pTM = 0.61 Download help

pTM is for the estimate of the TM-score, which is obtained from a pairwise error prediction. The higher pTM score indicates better model quality.

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence
90 < plddt <=100 ;
70 < plddt <= 90 ;
50 < plddt <= 70 ;
0 <= plddt <= 50 ;      Download help

Structural homologs

No homologs found in AlphaFold database

No homologs found in esmfold database

Family information      Download help

Fam ID Seed protein Member count Model Alignment
APIS005 APD21271.1 29 HMM model Member alignment

Host Taxa distribution

Length distribution

Homology to other families       download full data without filtering help

Clan ID: CLAN004

Hit APIS family E-Value Query Start Query End Hit Start Hit End Immune system Seed protein
APIS046 1.33E-82 7 320 2 326 IMGVR_UViG_3300009034_000088|3300009034|Ga0115863_100105265
APIS068 1.65E-82 7 319 2 330 IMGVR_UViG_3300035698_004593|3300035698|Ga0374944_618332_7825_8844
APIS083 1.29E-64 5 319 20 341 CP067347_00056
APIS088 1.12E-61 5 320 3 303 uvig_218805_40
APIS065 4.57E-54 5 320 3 341 KJ018209_00009
APIS071 3.55E-51 5 319 2 336 IMGVR_UViG_3300037628_000643|3300037628|Ga0310922_0000037_90720_91751
APIS053 5.80E-46 15 319 87 374 IMGVR_UViG_3300014203_000236|3300014203|Ga0172378_100030802
APIS057 1.11E-36 50 320 44 320 IMGVR_UViG_3300021167_000128|3300021167|Ga0216365_13592277

Family members, sources, and their hosts        help

Results of representative protein blast against members.

Member pident evalue bitscore qcovs phage host SourceDB Pfam Phrog
APD21271.1 100.000 0.0 601 100% phi3T of B. subtilis Bacillus subtilis NCBI phrog_20113
IMGVR_UViG_2541047086_000003|2541047086|2541780738 93.559 0.0 573 100% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2627854101_000001|2627854101|2630228600 93.898 0.0 575 100% Bacillus siamensis IMGVR phrog_20113
IMGVR_UViG_2660238538_000004|2660238538|2662791352 95.254 0.0 580 100% Bacillus subtilis IMGVR phrog_20113
IMGVR_UViG_2663763031_000002|2663763031|2665375316 99.661 0.0 600 100% Bacillus subtilis IMGVR phrog_20113
IMGVR_UViG_2667527573_000005|2667527573|2668626147 95.254 0.0 581 100% Bacillus subtilis IMGVR phrog_20113
IMGVR_UViG_2718217648_000002|2718217648|2718348907 93.220 0.0 569 100% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2721755777_000003|2721755777|2723934697 94.915 0.0 578 100% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2721755807_000001|2721755807|2724030275 74.237 5.20e-162 461 100% Bacillus xiamenensis IMGVR phrog_20113
IMGVR_UViG_2724679183_000002|2724679183|2725786969 90.508 0.0 554 100% Bacillus nakamurai IMGVR phrog_20113
IMGVR_UViG_2791355488_000010|2791355488|2794455659 62.034 7.76e-134 389 100% Margalitia camelliae IMGVR phrog_20113
IMGVR_UViG_2806311071_000002|2806311071|2808605495 92.517 0.0 563 99% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2867919565_000004|2867919565|2867921145 94.915 0.0 581 100% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2880152234_000004|2880152234|2880154041 93.898 0.0 572 100% Bacillus vallismortis IMGVR phrog_20113
IMGVR_UViG_2897118514_000003|2897118514|2897120904 90.847 0.0 556 100% Bacillus subtilis IMGVR phrog_20113
IMGVR_UViG_2904560550_000001|2904560550|2904561078 93.559 0.0 566 100% Bacillus velezensis IMGVR phrog_20113
IMGVR_UViG_2907202186_000003|2907202186|2907205396 50.169 3.30e-93 286 99% Paenibacillus sp009807035 IMGVR phrog_20113
IMGVR_UViG_2908369612_000002|2908369612|2908372509 89.492 0.0 551 100% Bacillus atrophaeus IMGVR phrog_20113
IMGVR_UViG_2912844605_000003|2912844605|2912847088 94.576 0.0 573 100% Bacillus subtilis IMGVR phrog_20113
IMGVR_UViG_2922727739_000003|2922727739|2922732230 48.276 1.19e-92 285 98% Paenibacillus polymyxa_C IMGVR phrog_20113
IMGVR_UViG_2938655777_000006|2938655777|2938662204 49.141 1.33e-92 285 99% Paenibacillus polymyxa_B IMGVR phrog_20113
IMGVR_UViG_3300005661_000004|3300005661|Ga0058698_1000002745 97.306 0.0 585 100% Bacillus IMGVR phrog_20113
IMGVR_UViG_3300025012_000028|3300025012|Ga0209727_1000023380 40.323 1.62e-72 234 99% IMGVR phrog_20113
IMGVR_UViG_3300025600_000004|3300025600|Ga0209125_100025782 44.816 2.54e-88 274 99% IMGVR phrog_20113
IMGVR_UViG_3300027858_000004|3300027858|Ga0209013_10000004121 56.271 6.64e-117 346 100% IMGVR phrog_20113
IMGVR_UViG_3300030692_000004|3300030692|Ga0268250_1000017453 97.306 0.0 585 100% Bacillus IMGVR phrog_20113
IMGVR_UViG_3300042922_000886|3300042922|Ga0456363_0000355_108865_109758 49.141 1.10e-88 275 99% IMGVR phrog_20113
OM236515_00131 97.288 0.0 588 100% Bacillus phage SPR Bacillus INPHARED phrog_20113
ON107264_00064 96.949 0.0 587 100% Bacillus phage BUCT083 Bacillus INPHARED phrog_20113