| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000173465 |
| Family | GT13 |
| Protein Properties | Length: 443 Molecular Weight: 51435.1 Isoelectric Point: 7.1245 |
| Chromosome | Chromosome/Scaffold: 002957519 Start: 11966 End: 16344 |
| Description | alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, putative |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT13 | 22 | 268 | 0 |
| IQIRLFTTQSKYADRLADAIESENQCTSQLRTLIDQISLQQGQIVSLEEEKQRLEQECGQLKVLVQELERKGLGRLIDKNQVPVAAVVIMACNRADYLER TVKSVLKYQSSVASKFPLFVSQDGSHPAVKQKALSYDQLTYMQHTRMAINSDFNELQHLDYEPVHAERPGELIAYYKIAXHYKWALDGLFYKHNFNRVII LEDDMEIAPDFFDYFEAAAALLDKDKSIMAVSSWNDNGQKQFVHDPK | |||
| GT13 | 283 | 435 | 0 |
| TYWDDWLRLKENHKGRQFIXPEVCRTYNFGEHGSSLGQFFKQYLEPIKLNDVPVDWKSMDLSYLMEDKYIKHFADIVQKAKPIRGSDLVLKARNIGGDVR VQYTDQQDFXYIASQFGIFEEWKDGVPRTAYKGIVVFRYHIPHRIFLVGPDSL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 443 Download |
| MKKILCDFRF LLFLAAAAFI YIQIRLFTTQ SKYADRLADA IESENQCTSQ LRTLIDQISL 60 QQGQIVSLEE EKQRLEQECG QLKVLVQELE RKGLGRLIDK NQVPVAAVVI MACNRADYLE 120 RTVKSVLKYQ SSVASKFPLF VSQDGSHPAV KQKALSYDQL TYMQHTRMAI NSDFNELQHL 180 DYEPVHAERP GELIAYYKIA XHYKWALDGL FYKHNFNRVI ILEDDMEIAP DFFDYFEAAA 240 ALLDKDKSIM AVSSWNDNGQ KQFVHDPKFR SISSKCFLSQ CFTYWDDWLR LKENHKGRQF 300 IXPEVCRTYN FGEHGSSLGQ FFKQYLEPIK LNDVPVDWKS MDLSYLMEDK YIKHFADIVQ 360 KAKPIRGSDL VLKARNIGGD VRVQYTDQQD FXYIASQFGI FEEWKDGVPR TAYKGIVVFR 420 YHIPHRIFLV GPDSLKQLGV NDS 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00761 | Glyco_tranf_GTA_type | 0.008 | 108 | 157 | 50 | + Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities. | ||
| cd02514 | GT13_GLCNAC-TI | 2.0e-140 | 105 | 434 | 350 | + GT13_GLCNAC-TI is involved in an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GLCNAC-T I , GNT-I) transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide, an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localized to the Golgi apparatus. The catalytic domain is located at the C-terminus. These proteins are members of the glycosy transferase family 13. | ||
| pfam03071 | GNT-I | 0 | 20 | 442 | 444 | + GNT-I family. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GNT-I, GLCNAC-T I) EC:2.4.1.101 transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide. This is an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localised to the Golgi apparatus, and is probably distributed in all tissues. The catalytic domain is located at the C-terminus. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0000139 | Golgi membrane |
| GO:0003827 | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity |
| GO:0006487 | protein N-linked glycosylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAC80697.1 | 0 | 3 | 443 | 5 | 446 | N-acetylglucosaminyltransferase I [Solanum tuberosum] |
| EMBL | CAC80700.1 | 0 | 1 | 443 | 1 | 444 | N-acetylglucosaminyltransferase I [Arabidopsis thaliana] |
| EMBL | CAC80702.1 | 0 | 3 | 443 | 5 | 446 | N-acetylglucosaminyltransferase I [Nicotiana tabacum] |
| EMBL | CBI29533.1 | 0 | 1 | 441 | 1 | 439 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_195537.2 | 0 | 1 | 443 | 1 | 444 | CGL1 (COMPLEX GLYCAN LESS 1); alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase/ protein N-acetylglucosaminyltransferase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1foa_A | 0 | 102 | 434 | 5 | 339 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 1fo9_A | 0 | 102 | 434 | 5 | 339 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 2apc_A | 0 | 105 | 434 | 2 | 333 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 2am5_A | 0 | 105 | 434 | 2 | 333 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 2am4_A | 0 | 105 | 434 | 2 | 333 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
