Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0002 enzyme activity assay, Northern Blot lichenan Bacillus subtilis 8606172
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J Bacteriol. 1996 Apr;178(7):1971-9. doi: 10.1128/jb.178.7.1971-1979.1996.
1996 Apr degradation 2 1 GH16
PUL0003 RT-PCR xylan Bacillus subtilis 26559526
Metabolic potential of Bacillus subtilis 168 for the direct conversion of xylans to fermentation products. Appl Microbiol Biotechnol. 2016 Feb;100(3):1501-1510. doi: 10.1007/s00253-015-7124-x. Epub 2015 Nov 12.
2016 Feb degradation 2 2 GH30, GH30_8, GH43_16, CBM6
PUL0019 enzyme activity assay, Northern Blot lichenan, cellobiose, beta-glucoside Bacillus subtilis 8990303
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis. J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997.
1997 Jan degradation 6 1 GH4
PUL0058 enzyme activity assay glycogen Bacillus subtilis 8145641
Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation. Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.
1994 Jan biosynthesis 5 3 GH13, CBM48, GH13_9, GT5, GT35
PUL0082 electrophoretic mobility shift assay, enzyme activity assay melibiose, raffinose-oligosaccharide, stachyose Bacillus subtilis 31138628
The melREDCA Operon Encodes a Utilization System for the Raffinose Family of Oligosaccharides in Bacillus subtilis. J Bacteriol. 2019 Jul 10;201(15):e00109-19. doi: 10.1128/JB.00109-19. Print 2019 Aug 1.
2019 Aug 1 degradation 6 2 GH4
PUL0238 Northern Blot glucomannan Bacillus subtilis 18177310
Glucomannan utilization operon of Bacillus subtilis. FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x.
2008 Feb degradation 8 1 GH26
PUL0249 microarray rhamnogalacturonan Bacillus subtilis 17449691
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. Appl Environ Microbiol. 2007 Jun;73(12):3803-13. doi: 10.1128/AEM.00147-07. Epub 2007 Apr 20.
2007 Jun degradation 12 6 GH105, CE12, PL11_1, CE12, PL11, PL11_1, PL11, CE12, GH42
PUL0323 fitness contribution assay, insertion sequencing galactan Bacillus subtilis subsp. subtilis 28617843
Characterization of the regulation of a plant polysaccharide utilization operon and its role in biofilm formation in Bacillus subtilis. PLoS One. 2017 Jun 15;12(6):e0179761. doi: 10.1371/journal.pone.0179761. eCollection 2017.
2017 degradation 6 2 CBM61, GH53, GH42
PUL0466 clone and expression, enzyme activity assay, Northern Blot arabinan Bacillus subtilis 14973026
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis. J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004.
2004 Mar degradation 9 1 GH51
PUL0569 clone and expression, enzyme activity assay, Northern Blot levan Bacillus subtilis 11739774
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
2001 Dec degradation 3 1 GH68, GH32
PUL0591 growth assay, Northern Blot N-acetylglucosamine Bacillus subtilis 23667565
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine. PLoS One. 2013 May 8;8(5):e63025. doi: 10.1371/journal.pone.0063025. Print 2013.
2013 degradation 4 1 CE9