| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi2g06030.1 |
| Family | AA7 |
| Protein Properties | Length: 565 Molecular Weight: 60197.8 Isoelectric Point: 5.2492 |
| Chromosome | Chromosome/Scaffold: 2 Start: 4532028 End: 4535620 |
| Description | cytokinin oxidase 5 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 77 | 270 | 2.6e-22 |
| AAPQAVLYPSSPGDIAALVRASWARAPASPFPVSARGRGHSTRGQAAAPGGVVVDMPSLGRGPGGTAERLSVSADGGYVDAGGEQLWVDVLHAALAHGLT PRSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVLQLDVITGVGEMVTCSKEERADLFEAVLGGLGQFGVITRARIRLAPAPARARWVRLLYT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 565 Download |
| MAVEAQALVT MVAVFLLSSF LTKSAIPEAA AGGGSSFLDE LRDALGDDAP ALVLRDDAEA 60 TARASADFGN VSSPVFAAPQ AVLYPSSPGD IAALVRASWA RAPASPFPVS ARGRGHSTRG 120 QAAAPGGVVV DMPSLGRGPG GTAERLSVSA DGGYVDAGGE QLWVDVLHAA LAHGLTPRSW 180 TDYLHLTVGG TLSNAGISGQ AFRHGPQISN VLQLDVITGV GEMVTCSKEE RADLFEAVLG 240 GLGQFGVITR ARIRLAPAPA RARWVRLLYT TAASLTGDQE RLIADGGGVS GLLDYVEGSV 300 LLADQLAGSW RSPGSSFSSD ADSGARVQAL AREAGGVLYC LEGALYYGGA SADGESDVAK 360 RLEVLLRELR YERGFAFVHD VPYSGFLDRV HSGELSLRAK GLWDVPHPWL NLFLPRSHIL 420 DFAAGVFHGI LRRDNDNHIT GAGGPVLIYP MNRDKWDEKM SAVFPEEEEV FYTVGILPSA 480 PPATSTDGGD ELRRMEEQNE EIMRFCEAAG IPCVQYLSYY AGQDGWEKKH FGPARWARFV 540 ERKRQYDPKA ILSRGQRIFT SPLA* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 1.0e-18 | 40 | 249 | 216 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 7.0e-23 | 79 | 227 | 150 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 7.0e-113 | 264 | 559 | 298 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 5 | 559 | 556 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB56095.1 | 0 | 43 | 564 | 30 | 558 | putative cytokinin oxidase [Oryza sativa Japonica Group] |
| DDBJ | BAE16612.1 | 0 | 6 | 564 | 3 | 565 | cytokinin oxidase/dehydrogenase [Oryza sativa Japonica Group] |
| GenBank | EEC70112.1 | 0 | 6 | 564 | 3 | 563 | hypothetical protein OsI_00771 [Oryza sativa Indica Group] |
| GenBank | EEE54051.1 | 0 | 6 | 564 | 3 | 525 | hypothetical protein OsJ_00744 [Oryza sativa Japonica Group] |
| RefSeq | XP_002455003.1 | 0 | 38 | 564 | 50 | 573 | hypothetical protein SORBIDRAFT_03g002810 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 54 | 560 | 29 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 54 | 560 | 29 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 54 | 560 | 29 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 54 | 560 | 29 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 54 | 560 | 29 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
