| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0001 | RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 7 | GH1, CE2, GH130, GH130, GH36, GH113 |
| PUL0099 | RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 3 | 2 | CBM27, GH26, CBM23, GH3, GH3 |
| PUL0130 | NMR, mass spectrometry | O-antigen | Yersinia rohdei | 30385338 Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78. Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29. |
2019 Feb 1 | biosynthesis | 11 | 3 | GT4, GT4 |
| PUL0160 | mass spectrometry, sequence homology analysis | alpha-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 33 | 10 | GH92, GH92, GH43, GH43_34, GH125, CBM32, GH92, GH92, GH92, GH76, GH2, GH92 |
| PUL0161 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 26 | 6 | GH97, GH67, GH76, GH92, GH76, GH125 |
| PUL0162 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 13 | 2 | GH92, GH99 |
| PUL0163 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 21 | 7 | GH92, GH38, CBM32, GT32, GT32, GH130, GH125, GH76, GH92, GH76 |
| PUL0164 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Leeuwenhoekiella sp. MAR_2009_132 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 19 | 9 | CE2, GH3, GH5_7, GH26, GH130, GH26, GH5_2, GH5, GH27, GH9, GH26 |
| PUL0165 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 17 | 6 | GH9, GH27, GH5, GH5_2, GH26, GH130, GH26 |
| PUL0167 | mass spectrometry, sequence homology analysis | beta-mannan | Bacteroides ovatus | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 12 | 4 | GH130, GH36, GH26, GH26 |
| PUL0220 | mass spectrometry, target decoy database analysis | laminarin | Polaribacter sp. Hel1_33_49 | 25478683 Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5. |
2015 Jun | degradation | 11 | 5 | GH30, GH30_1, GH17, GH3, GH149, GH16 |
| PUL0234 | proteome fractionation, mass spectrometry, target decoy database analysis | laminarin | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 7 | 3 | GH16, GH3, GH16 |
| PUL0235 | proteome fractionation, mass spectrometry, target decoy database analysis | alginate | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 20 | 7 | PL7, PL7_5, PL6_1, PL6, PL7, PL7_5, PL17, PL17_2, PL7, PL7 |
| PUL0236 | proteome fractionation, mass spectrometry, target decoy database analysis | alpha-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 13 | 4 | GH13, GH13, GH13_36, GH65, GH13_7 |
| PUL0286 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Providencia alcalifaciens | 23163869 Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. FEMS Immunol Med Microbiol. 2012 Dec;66(3):382-92. doi: 10.1111/1574-695X.12002. |
2012 Dec | biosynthesis | 16 | 4 | GT2, GT4, GT2 |
| PUL0288 | acid hydrolysis, NMR, mass spectrometry, sugar utilization assay | capsule polysaccharide, heparosan | Escherichia coli | 22975275 Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res. 2012 Oct 1;360:19-24. doi: 10.1016/j.carres.2012.07.013. Epub 2012 Jul 27. |
2012 Oct 1 | biosynthesis | 5 | 1 | GT2 |
| PUL0290 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Yersinia intermedia | 29524727 Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17). Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28. |
2018 May 2 | degradation | 12 | 4 | GT4, GT4 |
| PUL0308 | microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq | beta-glucan | Bacteroides ovatus | 29020628, 22205877, 32801182 Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. |
2017 Oct 10,2011 Dec,2020 Oct 1 | degradation | 5 | 3 | GH16, GH3 |
| PUL0320 | liquid chromatography and mass spectrometry, mass spectrometry, target decoy database analysis | glucan | Caldicellulosiruptor bescii | 29475869, 29588665 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. Biotechnol Biofuels. 2018 Mar 23;11:80. doi: 10.1186/s13068-018-1076-1. eCollection 2018. |
2018 May 1,2018 | degradation | 19 | 9 | CE12, PL11, CBM3, PL3_1, CBM66, PL3, PL9_1, PL9, CBM66, CBM22, GH10, GH48, CBM3, GH44, GH5, CBM3, GH5_8, GH48, GH74, CBM3, GT39, GH5, GH9, CBM3, GH5_8, GH5_1, CBM3, GH5_8, GH9, GH48, CBM3 |
| PUL0367 | mass spectrometry | galactooligosaccharide | Bifidobacterium longum | 18539808 Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide. Appl Environ Microbiol. 2008 Aug;74(15):4686-94. doi: 10.1128/AEM.00122-08. Epub 2008 Jun 6. |
2008 Aug | degradation | 3 | 1 | GH42 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 12 | 10 | GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1 |
| PUL0386 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | hemicellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 11 | 11 | GH43, CBM6, GH43_16, GH10, CBM6, GH43_29, GH43, CBM6, CE1, CBM6, GH43_10, CBM6, GH62, CBM6, GH43_29, GH43, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6 |
| PUL0435 | mass spectrometry, high performance anion exchange chromatography | glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 13 | 4 | GH43, GH43_34, GH30, GH30_1, GH30, GH30_3, GH16 |
| PUL0436 | mass spectrometry, high performance anion exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 8 | 2 | GH65, GH13, CBM32 |
| PUL0437 | mass spectrometry, high performance anion exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 1 | GH51 |
| PUL0438 | mass spectrometry, high performance anion exchange chromatography | glucomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 7 | 3 | GH89, GH158 |
| PUL0439 | mass spectrometry, high performance anion exchange chromatography | glucomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 4 | 1 | GH33 |
| PUL0440 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 5 | 2 | GH2, GH76, CBM13 |
| PUL0441 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH130, CBM13, GH43_26 |
| PUL0442 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | CBM5, GH18, GH18, GH64, CBM6, GH16, CBM6 |
| PUL0443 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | GH16, CBM6 |
| PUL0444 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH31, GH19, CBM5 |
| PUL0627 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii LUH5543 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 21 | 1 | GT2 |
| PUL0628 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_204 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 2 | GT2, GT2, GT2, GT2 |
| PUL0629 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_309 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 1 | GT2, GT2 |
| PUL0648 | high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization | glucuronoarabinoxylan | Dysgonomonas mossii DSM 22836 | 33667545 A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2. |
2021 Jan-Jun | degradation | 37 | 20 | CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67 |
| PUL0652 | RNA-Seq, enzyme activity assay, thin layer chromatography, liquid chromatography, mass spectrometry | agar | Colwellia echini A3 | 33811026 A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26. |
2021 May 26 | degradation | 54 | 10 | CBM35, GH2, CBM6, GH96, CBM6, GH96, GH50, GH50, GH50, GH29, CBM13, GH50, GH86, GH117 |
| PUL0670 | NMR, Smith degradation, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii KZ-1098 | 34537298 The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase. Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16. |
2021 Nov 30 | biosynthesis | 24 | 2 | GT2, GT4 |
| PUL0672 | Smith degradation, NMR, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii Ab-46-1632 | 34757131 Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis. Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30. |
2021 Dec 15 | biosynthesis | 20 | 4 | GT2, GT2, GT2, GT2 |
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