| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0025 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 32093600 Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549. Biochemistry (Mosc). 2020 Feb;85(2):241-247. doi: 10.1134/S000629792002011X. |
2020 Feb | biosynthesis | 18 | 4 | GT2, GT4, GT0, GT2 |
| PUL0043 | Smith degradation, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 29886169 Structure and gene cluster of the K125 capsular polysaccharide from Acinetobacter baumannii MAR13-1452. Int J Biol Macromol. 2018 Oct 1;117:1195-1199. doi: 10.1016/j.ijbiomac.2018.06.029. Epub 2018 Jun 7. |
2018 Oct 1 | biosynthesis | 23 | 3 | GT4 |
| PUL0046 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29738687 O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104. Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061. |
2018 May | biosynthesis | 16 | 5 | GT2 |
| PUL0047 | sugar utilization assay, NMR, sequence homology analysis | O-antigen | Escherichia albertii | 31622726 Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway. Int J Biol Macromol. 2020 Jan 1;142:609-614. doi: 10.1016/j.ijbiomac.2019.09.135. Epub 2019 Oct 14. |
2020 Jan 1 | biosynthesis | 25 | 8 | GT4, GT4 |
| PUL0057 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29787897 Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7. |
2018 Jul 15 | biosynthesis | 13 | 5 | GT4, GT4 |
| PUL0059 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29787897 Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7. |
2018 Jul 15 | biosynthesis | 13 | 3 | GT2 |
| PUL0060 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 24 | 4 | GT2, GT2, GT4, GT2 |
| PUL0061 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 35 | 4 | GT2, GT2, GT4, GT2, GT4, CE4 |
| PUL0062 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 19 | 4 | GT2, GT2, GT4, GT2 |
| PUL0069 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT0, GT2 |
| PUL0070 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 36 | 3 | GT52, GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0071 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 38 | 4 | GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0072 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT2, GT0 |
| PUL0073 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT2 |
| PUL0090 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 31220629 Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6. |
2019 Jul 15 | biosynthesis | 12 | 4 | GT4, GT4, GT4 |
| PUL0130 | NMR, mass spectrometry | O-antigen | Yersinia rohdei | 30385338 Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78. Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29. |
2019 Feb 1 | biosynthesis | 11 | 3 | GT4, GT4 |
| PUL0172 | gene deletion mutant and growth assay, NMR, sugar utilization assay | O-antigen | Franconibacter pulveris | 27166227 O antigen of FranconibacterpulverisG3872 (O1) is a 4-deoxy-d-arabino-hexose-containing polysaccharide synthesized by the ABC-transporter-dependent pathway. Microbiology (Reading). 2016 Jul;162(7):1103-1113. doi: 10.1099/mic.0.000307. Epub 2016 May 10. |
2016 Jul | biosynthesis | 9 | 3 | GT2, GT2 |
| PUL0173 | sequence homology analysis, NMR, sugar utilization assay | O-antigen | Escherichia coli | 27177202 Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal. Carbohydr Res. 2016 Jul 22;430:24-28. doi: 10.1016/j.carres.2016.04.025. Epub 2016 Apr 29. |
2016 Jul 22 | biosynthesis | 20 | 4 | GT4, GT4, GT2 |
| PUL0177 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27293097 Structure and Biosynthesis Gene Cluster of the O-Antigen of Escherichia coli O12. Biochemistry (Mosc). 2016 Apr;81(4):401-6. doi: 10.1134/S0006297916040106. |
2016 Apr | biosynthesis | 15 | 4 | GT4 |
| PUL0181 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27083849 Structure of the beta-l-fucopyranosyl phosphate-containing O-specific polysaccharide of Escherichia coli O84. Int J Biol Macromol. 2016 Jul;88:578-85. doi: 10.1016/j.ijbiomac.2016.04.025. Epub 2016 Apr 13. |
2016 Jul | biosynthesis | 19 | 5 | GT4, GT2 |
| PUL0182 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 12 | 3 | GT2 |
| PUL0183 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 15 | 3 | GT2 |
| PUL0184 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 20 | 3 | GT4 |
| PUL0188 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26582605 Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid. Glycobiology. 2016 Apr;26(4):335-42. doi: 10.1093/glycob/cwv106. Epub 2015 Nov 17. |
2016 Apr | biosynthesis | 10 | 3 | GT0, GT2 |
| PUL0198 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26706815 Structure and gene cluster of the o-antigen of Escherichia coli o96. Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23. |
2016 Feb | biosynthesis | 13 | 6 | GT2, GT2 |
| PUL0200 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26451883 Structure and genetics of biosynthesis of the glycosyl phosphate-containing O-polysaccharide of Escherichia coli O160. Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16. |
2015 Nov 19 | biosynthesis | 13 | 4 | GT2, GT2 |
| PUL0201 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26382081 Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170. Carbohydr Res. 2015 Nov 19;417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28. |
2015 Nov 19 | biosynthesis | 13 | 4 | GT2 |
| PUL0202 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26342864 Structure and gene cluster of the O-antigen of Escherichia coli O43. Carbohydr Res. 2015 Oct 30;416:32-6. doi: 10.1016/j.carres.2015.08.008. Epub 2015 Aug 18. |
2015 Oct 30 | biosynthesis | 19 | 4 | GT4, GT2 |
| PUL0286 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Providencia alcalifaciens | 23163869 Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. FEMS Immunol Med Microbiol. 2012 Dec;66(3):382-92. doi: 10.1111/1574-695X.12002. |
2012 Dec | biosynthesis | 16 | 4 | GT2, GT4, GT2 |
| PUL0288 | acid hydrolysis, NMR, mass spectrometry, sugar utilization assay | capsule polysaccharide, heparosan | Escherichia coli | 22975275 Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res. 2012 Oct 1;360:19-24. doi: 10.1016/j.carres.2012.07.013. Epub 2012 Jul 27. |
2012 Oct 1 | biosynthesis | 5 | 1 | GT2 |
| PUL0290 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Yersinia intermedia | 29524727 Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17). Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28. |
2018 May 2 | degradation | 12 | 4 | GT4, GT4 |
| PUL0301 | sugar utilization assay, NMR | O-antigen | Escherichia coli | 29309918 Structure elucidation of the O-specific polysaccharide by NMR spectroscopy and selective cleavage and genetic characterization of the O-antigen of Escherichia albertii O5. Carbohydr Res. 2018 Mar 2;457:25-31. doi: 10.1016/j.carres.2017.12.010. Epub 2018 Jan 3. |
2018 Mar 2 | biosynthesis | 10 | 3 | GT32, GT2, GT2 |
| PUL0388 | sugar utilization assay, NMR | O-antigen | Salmonella enterica | 20501518 Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25. |
2011 Apr | biosynthesis | 9 | 4 | GT4, GT4, GT2 |
| PUL0389 | sugar utilization assay, NMR | O-antigen | Escherichia coli | 20501518 Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25. |
2011 Apr | biosynthesis | 8 | 4 | GT4, GT4, GT2 |
| PUL0431 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27454490 Structure elucidation and gene cluster characterization of the O-antigen of Escherichia coli O80. Carbohydr Res. 2016 Sep 2;432:83-7. doi: 10.1016/j.carres.2016.07.011. Epub 2016 Jul 14. |
2016 Sep 2 | biosynthesis | 18 | 4 | GT26 |
| PUL0432 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27645300 Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19. |
2017 Feb | biosynthesis | 11 | 3 | GT4, GT2 |
| PUL0433 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27645300 Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19. |
2017 Feb | biosynthesis | 12 | 3 | GT2 |
| PUL0446 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28494314 Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5. Carbohydr Res. 2017 Jun 29;446-447:28-31. doi: 10.1016/j.carres.2017.04.024. Epub 2017 May 2. |
2017 Jun 29 | biosynthesis | 17 | 2 | GT4 |
| PUL0447 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 14 | 4 | GT4, GT4 |
| PUL0448 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 19 | 4 | GT4 |
| PUL0449 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 13 | 4 | GT4, GT2, GT4 |
| PUL0450 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 14 | 3 | GT52, GT2, GT2 |
| PUL0451 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4 |
| PUL0452 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4, GT2 |
| PUL0453 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4 |
| PUL0461 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Yersinia kristensenii | 31220629 Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6. |
2019 Jul 15 | biosynthesis | 12 | 4 | GT4, GT4, GT4 |
| PUL0462 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 24607538 Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages. Carbohydr Res. 2014 Mar 31;388:30-6. doi: 10.1016/j.carres.2014.02.013. Epub 2014 Feb 18. |
2014 Mar 31 | biosynthesis | 14 | 4 | GT0, GT4, GT2 |
| PUL0582 | NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay | lactose | Lactococcus lactis | 22660716 A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1. |
2012 Aug | degradation | 5 | 1 | GH1 |
| PUL0627 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii LUH5543 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 21 | 1 | GT2 |
| PUL0628 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_204 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 2 | GT2, GT2, GT2, GT2 |
| PUL0629 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_309 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 1 | GT2, GT2 |
| PUL0648 | high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization | glucuronoarabinoxylan | Dysgonomonas mossii DSM 22836 | 33667545 A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2. |
2021 Jan-Jun | degradation | 37 | 20 | CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67 |
| PUL0649 | Smith degradation, NMR | capsule polysaccharide | Acinetobacter baumannii B8300 | 33667610 A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300. Res Microbiol. 2021 Apr-May;172(3):103815. doi: 10.1016/j.resmic.2021.103815. Epub 2021 Mar 3. |
2021 Apr-May | biosynthesis | 22 | 1 | GT2, GT2 |
| PUL0650 | enzyme activity assay, high performance anion exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay | arabinogalactan | Bifidobacterium longum JCM 7052 | 33674431 Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27. |
2021 Apr 27 | degradation | 7 | 2 | GH36, GH39, CBM35 |
| PUL0651 | enzyme activity assay, NMR | agar | Gilvimarinus chinensis DSM 19667 | 33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 14 | CBM6, GH16_16, GH16, GH50, CBM6, CBM13, CBM6, GH16_16, GH16, GH50, GH117, GH16_3, GH16, GH2, GH50, GH127, GH167, CBM2, CBM6, GH86, 3.2.1.81, GH50, 3.2.1.81, GH86 |
| PUL0657 | recombinant protein expression, NMR | levoglucosan | Bacillus smithii S-2701M | 33208778 Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction. Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8. |
2020 Nov 18 | degradation | 8 | 2 | GH109, GH109 |
| PUL0660 | NMR, Smith degradation | capsule polysaccharide | Acinetobacter baumannii 48-1789 | 34073255 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641. |
2021 May 26 | biosynthesis | 21 | 2 | GT2, GT4 |
| PUL0661 | NMR, Smith degradation | capsule polysaccharide | Acinetobacter baumannii MAR-24 | 34073255 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641. |
2021 May 26 | biosynthesis | 22 | 2 | GT2, GT4 |
| PUL0670 | NMR, Smith degradation, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii KZ-1098 | 34537298 The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase. Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16. |
2021 Nov 30 | biosynthesis | 24 | 2 | GT2, GT4 |
| PUL0672 | Smith degradation, NMR, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii Ab-46-1632 | 34757131 Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis. Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30. |
2021 Dec 15 | biosynthesis | 20 | 4 | GT2, GT2, GT2, GT2 |
| PUL0673 | NMR, substrate binding assay, liquid chromatography and mass spectrometry | human milk oligosaccharide | Bifidobacterium pseudocatenulatum DSM20438 | 34757822 Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10. |
2022 Jan 25 | degradation | 8 | 1 | GH95 |
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