| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si000306m |
| Family | GT35 |
| Protein Properties | Length: 838 Molecular Weight: 94241.6 Isoelectric Point: 7.1671 |
| Chromosome | Chromosome/Scaffold: 5 Start: 41621045 End: 41627332 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 109 | 832 | 0 |
| AVKKFGYELEVLAGQEKDAALGNGGLGRLASCFLDSMATLNLPAWGYGLRYRYGLFKQHIAKEGQEETAEDWLDKFSPWEIPRHDVVFPVRFFGHVEILP DGSRKWVGGEVLKALAYDVPIPGYKTKNAISLRLWEAKATAEDFNLFQFNDGQYESAAQLHARAQQICAVLYPGDATEEGKLLRLKQQFFLCSASLQDMI ARFKERKADRASGKWSEFPTKVAVQLNDTHPTLAIPELMRLLMDEEGLGWDEAWDITYRTVSYTNHTVLPEALEKWSQIVMRKLLPRHMEIIEEIDKRFR ELVISKHKEMEGKIDSMKVLDSSNPQKPVVRMANLCVVSSHTVNGVAELHSNILKQELFADYVSIWPNKFQNKTNGITPRRWLKFCNPELSEIVTKWLKT DQWTSNLDLLTGLRKFADDEKLHAEWAAAKLACKKRLAKHVLDVTGVTIDPTSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKEMSAEEKQKVTPRTVML GGKAFATYTNAKRIVKLVNDVGAVVNNDPEVNKYLKVVFIPNYNVSVAEVLIPGSELSQHISTAGMEASGTSNMKFSLNGCVIIGTLDGANVEIREEVGE DNFFLFGAKADEVAGLRKDRENGLFKPDPRFEEAKQLIRSGAFGSYNYEPLLDSLEGNSGFGRGDYFLVGYDFPGYIDAQDRVDAAYKDKKKWIKMSILN TAGSGKFSSDRTIAQYAKEIWDIK | |||
| Full Sequence |
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| Protein Sequence Length: 838 Download |
| MPEKCGAAEK VKPAASPAAE KPADIAGNIS YHAQYSPHFS PLAFGPEEAF YATAESVRDH 60 LIERWNDTYV HFHKTDPKQT YYLSMEYLQG RALTNAVGNL GITGAYAEAV KKFGYELEVL 120 AGQEKDAALG NGGLGRLASC FLDSMATLNL PAWGYGLRYR YGLFKQHIAK EGQEETAEDW 180 LDKFSPWEIP RHDVVFPVRF FGHVEILPDG SRKWVGGEVL KALAYDVPIP GYKTKNAISL 240 RLWEAKATAE DFNLFQFNDG QYESAAQLHA RAQQICAVLY PGDATEEGKL LRLKQQFFLC 300 SASLQDMIAR FKERKADRAS GKWSEFPTKV AVQLNDTHPT LAIPELMRLL MDEEGLGWDE 360 AWDITYRTVS YTNHTVLPEA LEKWSQIVMR KLLPRHMEII EEIDKRFREL VISKHKEMEG 420 KIDSMKVLDS SNPQKPVVRM ANLCVVSSHT VNGVAELHSN ILKQELFADY VSIWPNKFQN 480 KTNGITPRRW LKFCNPELSE IVTKWLKTDQ WTSNLDLLTG LRKFADDEKL HAEWAAAKLA 540 CKKRLAKHVL DVTGVTIDPT SLFDIQIKRI HEYKRQLLNI LGAVYRYKKL KEMSAEEKQK 600 VTPRTVMLGG KAFATYTNAK RIVKLVNDVG AVVNNDPEVN KYLKVVFIPN YNVSVAEVLI 660 PGSELSQHIS TAGMEASGTS NMKFSLNGCV IIGTLDGANV EIREEVGEDN FFLFGAKADE 720 VAGLRKDREN GLFKPDPRFE EAKQLIRSGA FGSYNYEPLL DSLEGNSGFG RGDYFLVGYD 780 FPGYIDAQDR VDAAYKDKKK WIKMSILNTA GSGKFSSDRT IAQYAKEIWD IKASPVA* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 28 | 831 | 812 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 29 | 831 | 811 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 109 | 832 | 729 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| PRK14985 | PRK14985 | 0 | 82 | 832 | 756 | + maltodextrin phosphorylase; Provisional | ||
| COG0058 | GlgP | 0 | 24 | 833 | 814 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EEC71819.1 | 0 | 1 | 837 | 1 | 841 | hypothetical protein OsI_04460 [Oryza sativa Indica Group] |
| RefSeq | NP_001044823.1 | 0 | 1 | 837 | 1 | 841 | Os01g0851700 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001151625.1 | 0 | 1 | 836 | 1 | 837 | LOC100285259 [Zea mays] |
| Swiss-Prot | Q9LKJ3 | 0 | 7 | 836 | 3 | 831 | PHSH_WHEAT RecName: Full=Alpha-glucan phosphorylase, H isozyme; AltName: Full=Starch phosphorylase H |
| RefSeq | XP_002458773.1 | 0 | 1 | 837 | 1 | 838 | hypothetical protein SORBIDRAFT_03g040060 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 18 | 834 | 21 | 831 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el5_A | 0 | 18 | 834 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el0_A | 0 | 18 | 834 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4eky_A | 0 | 18 | 834 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4eke_A | 0 | 18 | 834 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO782200 | 413 | 165 | 577 | 0 |
| HO418036 | 539 | 235 | 753 | 0 |
| GT044838 | 338 | 471 | 808 | 0 |
| GT043461 | 330 | 456 | 785 | 0 |
| GO883181 | 331 | 391 | 721 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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