| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g003186m |
| Family | GT35 |
| Protein Properties | Length: 841 Molecular Weight: 95249.5 Isoelectric Point: 7.2152 |
| Chromosome | Chromosome/Scaffold: 00026 Start: 391748 End: 398560 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 116 | 834 | 0 |
| ALNNLGHVLEEIAEQEKDAALGNGGLGRLASCFLDSMATLNLPAWGYGLRYRYGLFKQKITKQGQEEVAEDWLEKFSPWEVVRHDVVFPVRFFGSVMVNP NGTRKWVGGEVVQAVAYDIPIPGYKTKNTISLRLWDAKASAEDFNLFQFNDGQYESAAQLHSRAQQICAVLYPGDSTEEGKLLRLKQQFFLCSASLQDMI LRFKERKSGRQWSEFPSKVAVQLNDTHPTLAIPELMRLLMDEEGLGWDEAWDITTRTVAYTNHTVLPEALEKWSQAVMWKLLPRHMEIIEEIDKRFIAMV RSTRSDLESKIPSMCILDNNPKKPVVRMANLCVVSAHTVNGVAQLHSDILKADLFADYVSLWPNKLQNKTNGITPRRWLRFCNPELSKIITKWLKTDQWV TNLDLLVGLRQFADNTELQAEWESAKMASKKHLADYIWRVTGVTIDPNSLFDIQVKRIHEYKRQLLNILGAIYRYKKLKEMSPQERKKTTPRTIMIGGKA FATYTNAKRIVKLVNDVGEVVNTDPEVNSYLKVVFVPNYNVSVAELLIPGSELSQHISTAGMEASGTSNMKFSLNGCLIIGTLDGANVEIRQEIGEENFF LFGAVAEQVPKLRKEREDGLFKPDPRFEEAKQFIRSGAFGSYDYNPLLDSLEGNTGYGRGDYFLVGYDFPSYLEAQDRVDQAYKDQKKWLKMSILSTAGS GKFSSDRTIAQYAKEIWNI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 841 Download |
| MADAKANGKN EAAKLAKIPA AANPLANEPS AIASNISYHV QYSPHFSPTK FEPEQAFFAT 60 AESVRDRLIQ QWNETYHHFN KVDPKQTYYL SMEFLQGRTL TNAIGSLDIQ NAYADALNNL 120 GHVLEEIAEQ EKDAALGNGG LGRLASCFLD SMATLNLPAW GYGLRYRYGL FKQKITKQGQ 180 EEVAEDWLEK FSPWEVVRHD VVFPVRFFGS VMVNPNGTRK WVGGEVVQAV AYDIPIPGYK 240 TKNTISLRLW DAKASAEDFN LFQFNDGQYE SAAQLHSRAQ QICAVLYPGD STEEGKLLRL 300 KQQFFLCSAS LQDMILRFKE RKSGRQWSEF PSKVAVQLND THPTLAIPEL MRLLMDEEGL 360 GWDEAWDITT RTVAYTNHTV LPEALEKWSQ AVMWKLLPRH MEIIEEIDKR FIAMVRSTRS 420 DLESKIPSMC ILDNNPKKPV VRMANLCVVS AHTVNGVAQL HSDILKADLF ADYVSLWPNK 480 LQNKTNGITP RRWLRFCNPE LSKIITKWLK TDQWVTNLDL LVGLRQFADN TELQAEWESA 540 KMASKKHLAD YIWRVTGVTI DPNSLFDIQV KRIHEYKRQL LNILGAIYRY KKLKEMSPQE 600 RKKTTPRTIM IGGKAFATYT NAKRIVKLVN DVGEVVNTDP EVNSYLKVVF VPNYNVSVAE 660 LLIPGSELSQ HISTAGMEAS GTSNMKFSLN GCLIIGTLDG ANVEIRQEIG EENFFLFGAV 720 AEQVPKLRKE REDGLFKPDP RFEEAKQFIR SGAFGSYDYN PLLDSLEGNT GYGRGDYFLV 780 GYDFPSYLEA QDRVDQAYKD QKKWLKMSIL STAGSGKFSS DRTIAQYAKE IWNITECRTS 840 * 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 33 | 834 | 810 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 36 | 834 | 807 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 116 | 836 | 726 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| PRK14985 | PRK14985 | 0 | 89 | 832 | 750 | + maltodextrin phosphorylase; Provisional | ||
| COG0058 | GlgP | 0 | 29 | 834 | 810 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM29154.1 | 0 | 1 | 840 | 1 | 840 | starch phosphorylase type H [Citrus hybrid cultivar] |
| DDBJ | BAG31926.1 | 0 | 1 | 839 | 1 | 842 | alpha-1,4-glucan phosphorylase H isozyme [Cucurbita maxima] |
| RefSeq | XP_002280732.1 | 0 | 1 | 837 | 1 | 840 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002313399.1 | 0 | 4 | 839 | 14 | 852 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002520435.1 | 0 | 16 | 839 | 22 | 848 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 30 | 838 | 26 | 832 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1ygp_B | 0 | 56 | 834 | 68 | 875 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 56 | 834 | 68 | 875 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 4el5_A | 0 | 30 | 838 | 14 | 820 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 4el0_A | 0 | 30 | 838 | 14 | 820 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO782200 | 410 | 172 | 580 | 0 |
| HO418036 | 539 | 242 | 756 | 0 |
| HO586252 | 549 | 290 | 837 | 0 |
| HO613954 | 493 | 392 | 835 | 0 |
| EL450777 | 329 | 353 | 681 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
