| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0114 | recombinant protein expression, enzyme activity assay | arabinofuranose | Ruminiclostridium cellulolyticum | 31198441 The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-alpha-l-arabinofuranosidases with complementary modes of action. Biotechnol Biofuels. 2019 Jun 10;12:144. doi: 10.1186/s13068-019-1483-y. eCollection 2019. |
2019 | degradation | 14 | 14 | GH43, GH43_16, CBM6, GH10, CBM6, GH43, GH43_29, CBM6, CE1, CBM6, GH43_10, CBM6, GH62, CBM6, GH43, GH43_29, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6, GH62, CE6, CBM6, CBM32, GH95, CBM6, GH30_8, CBM6 |
| PUL0115 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 7 | 7 | GH33, GH20, GH2, GH20, GH20, GH2 |
| PUL0116 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 2 | 1 | GH20 |
| PUL0117 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 22 | 4 | GH92, GH130, GH163, GH20 |
| PUL0119 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 2 | 1 | CBM32, GH29 |
| PUL0120 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan, mucin | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 6 | 2 | CBM14, GH18 |
| PUL0151 | sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | 30524390, 32585009 Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2018,2020 Aug 20 | degradation | 12 | 2 | PL7, PL17_2, PL17 |
| PUL0203 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 7 | 3 | GH32, GH32, GH32 |
| PUL0204 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 7 | 3 | GH97 |
| PUL0205 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 6 | 3 | GH31, GH31, GH66 |
| PUL0211 | enzyme activity assay, gene deletion mutant and growth assay, thin layer chromatography | N-glycan | Xanthomonas campestris pv. campestris | 25586188, 25205095 The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14. |
2015 Mar 6,2014 Sep 9 | degradation | 9 | 6 | GH29, GH18, GH20, GH2, GH3, GH125, GH92, GH35 |
| PUL0230 | RT-PCR, enzyme activity assay, clone, enzyme kinetic analysis, thin layer chromatography, crystallization | maltooligosaccharide | Lactobacillus acidophilus | 22685275, 32444471 Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus. J Bacteriol. 2012 Aug;194(16):4249-59. doi: 10.1128/JB.00622-12. Epub 2012 Jun 8. Appl Environ Microbiol. 2020 Jul 20;86(15):e00661-20. doi: 10.1128/AEM.00661-20. Print 2020 Jul 20. |
2012 Aug,2020 Jul 20 | degradation | 12 | 4 | GH65, CBM34, GH13, GH13_20 |
| PUL0313 | microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | 28983288, 32585009 Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2017,2020 Aug 20 | degradation | 3 | 3 | PL6, PL6_1, PL7_5, PL7, PL6, PL6_1 |
| PUL0326 | gene deletion mutant and growth assay, enzyme activity assay, thin layer chromatography | beta-glucan | Bacteroides ovatus | 28461332 A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1. |
2017 Jun 23 | degradation | 13 | 2 | GH73 |
| PUL0330 | fosmid library screen, enzyme activity assay, thin layer chromatography | pectin | Gramella flava | 28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. |
2017 | degradation | 28 | 11 | CE8, PL9_1, GH28, GH105, GH43_10, GH28, PL9_1, CE12, CE8, CE10, CE12, PL10_1 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 9 | GH5, GH5_2, GH5_7, GH5, CE7, GH2, GH94, GH97, GH127, GH127 |
| PUL0333 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 22 | 3 | GH9, GH31, GH9 |
| PUL0334 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 23 | 5 | GH9, GH31, GH9, GH9, CE4 |
| PUL0335 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 31 | 4 | GH13, GH97, GH3, GH158, GH16 |
| PUL0336 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 25 | 3 | GH3, GH158, GH16 |
| PUL0337 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 29 | 3 | GH16, GH158, GH3 |
| PUL0338 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 34 | 4 | GH16, GH158, GH3, GH97 |
| PUL0339 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 24 | 4 | GH97, GH3, GH16, GH20 |
| PUL0340 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 37 | 1 | GH5_2, GH5 |
| PUL0341 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 43 | 3 | GH32, GH91, GH5, GH5_2 |
| PUL0353 | microarray, enzyme activity assay, strcutural analysis, clone and expression | arabinan, xylan, levan, pectin, rhamnogalacturonan | Bacteroides thetaiotaomicron | 16968696, 32060313 Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. Nat Commun. 2020 Feb 14;11(1):899. doi: 10.1038/s41467-020-14754-7. |
2006 Nov 24,2020 Feb 14 | degradation | 11 | 3 | GH0, GH92, GH92 |
| PUL0408 | enzyme activity assay, thin layer chromatography | beta-mannan | Bacteroides fragilis | 24217874 The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis. Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12. |
2014 Jan | degradation | 4 | 2 | GH26, GH130 |
| PUL0412 | clone and expression, enzyme activity assay | polygalacturonic acid | Ralstonia solanacearum | 12795379 Characterization of a Ralstonia solanacearum operon required for polygalacturonate degradation and uptake of galacturonic acid. Mol Plant Microbe Interact. 2003 Jun;16(6):536-44. doi: 10.1094/MPMI.2003.16.6.536. |
2003 Jun | degradation | 2 | 1 | GH28 |
| PUL0414 | enzyme activity assay, thin layer chromatography | xylan | uncultured bacterium 35A20 | 30116044 Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 28 | 7 | GH10, GH10 |
| PUL0423 | clone and expression, enzyme activity assay | cellobiose | Thermotoga neapolitana | 10960102 Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/JB.182.18.5172-5179.2000. |
2000 Sep | degradation | 3 | 2 | GH94 |
| PUL0445 | recombinant protein expression, thin layer chromatography, enzyme activity assay | alginate | Sphingomonas sp. | 10913091 Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000. |
2000 Aug | degradation | 8 | 2 | PL7, PL5, PL15_1 |
| PUL0455 | clone and expression, genes induced in presence of substrate, enzyme activity assay | sucrose | Bifidobacterium animalis | 12513973 Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose. Appl Environ Microbiol. 2003 Jan;69(1):24-32. doi: 10.1128/AEM.69.1.24-32.2003. |
2003 Jan | degradation | 3 | 1 | GH13, GH13_18 |
| PUL0460 | recombinant protein expression, RT-PCR, enzyme activity assay | carrageenan, agar | Paraglaciecola hydrolytica | 29774012 A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018. |
2018 | degradation | 108 | 18 | GH2, GH29, GH63, GH86, CBM6, GH50, GH86, GH50, GH117, GH50, GH16, GH50, CE1, GH16, GH127, GH16, GH82, GH16, GH16, GH16 |
| PUL0466 | clone and expression, enzyme activity assay, Northern Blot | arabinan | Bacillus subtilis | 14973026 Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis. J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004. |
2004 Mar | degradation | 9 | 1 | GH51 |
| PUL0475 | clone and expression, gene deletion mutant and growth assay | cellobiose, cellotriose | Streptomyces reticuli | 10347054 Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999. |
1999 Jun | degradation | 7 | 1 | GH18, CBM2 |
| PUL0497 | clone and expression, enzyme activity assay | chitin | Pseudoalteromonas piscicida | 11772635 Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7. Appl Environ Microbiol. 2002 Jan;68(1):263-70. doi: 10.1128/AEM.68.1.263-270.2002. |
2002 Jan | degradation | 3 | 2 | CBM5, GH18, CBM5, AA10, CBM5, GH18 |
| PUL0508 | clone and expression, enzyme activity assay | xylobiose, xylotriose | Streptomyces thermoviolaceus | 14761997 Molecular characterization of a high-affinity xylobiose transporter of Streptomyces thermoviolaceus OPC-520 and its transcriptional regulation. J Bacteriol. 2004 Feb;186(4):1029-37. doi: 10.1128/JB.186.4.1029-1037.2004. |
2004 Feb | degradation | 5 | 2 | GH3 |
| PUL0520 | clone and expression, enzyme activity assay | xylobiose, xylodextrin | Klebsiella oxytoca | 14532050 Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli. Appl Environ Microbiol. 2003 Oct;69(10):5957-67. doi: 10.1128/AEM.69.10.5957-5967.2003. |
2003 Oct | degradation | 2 | 1 | GH43, GH43_11 |
| PUL0531 | clone and expression, enzyme activity assay | chitobiose | Serratia marcescens | 12618440 Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170. J Bacteriol. 2003 Mar;185(6):1776-82. doi: 10.1128/JB.185.6.1776-1782.2003. |
2003 Mar | degradation | 5 | 1 | GH1 |
| PUL0561 | clone and expression, enzyme activity assay | alpha-galactoside | Lactobacillus plantarum | 12406739 Characterization of the melA locus for alpha-galactosidase in Lactobacillus plantarum. Appl Environ Microbiol. 2002 Nov;68(11):5464-71. doi: 10.1128/AEM.68.11.5464-5471.2002. |
2002 Nov | degradation | 5 | 2 | GH36, GH2 |
| PUL0567 | clone and expression, enzyme activity assay | chitin | Pseudoalteromonas sp. S9 | 10220172 Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91. Microbiology (Reading). 1999 Apr;145 ( Pt 4):925-934. doi: 10.1099/13500872-145-4-925. |
1999 Apr | degradation | 3 | 3 | CBM5, GH18, AA10, CBM5, CBM5, GH18 |
| PUL0568 | clone and expression, enzyme activity assay, Northern Blot | sucrose | Clostridium beijerinckii | 10411273 The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461. |
1999 Jun | degradation | 4 | 1 | GH32 |
| PUL0569 | clone and expression, enzyme activity assay, Northern Blot | levan | Bacillus subtilis | 11739774 yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413. |
2001 Dec | degradation | 3 | 1 | GH68, GH32 |
| PUL0570 | clone and expression, enzyme activity assay | cellobiose | Corynebacterium glutamicum | 12777497 A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0. |
2003 Jun | degradation | 3 | 1 | GH1 |
| PUL0589 | Western Blot, enzyme activity assay, thin layer chromatography | starch | Streptococcus mutans | 23930155 The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. doi: 10.3402/jom.v5i0.21285. Print 2013. |
2013 | degradation | 3 | 2 | GT35, GH77 |
| PUL0606 | enzyme activity assay, clone and expression | beta-galactooligosaccharide | Bifidobacterium breve UCC2003 | 32385941 Biochemical analysis of cross-feeding behaviour between two common gut commensals when cultivated on plant-derived arabinogalactan. Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9. |
2020 Nov | degradation | 3 | 1 | 3.2.1.23, GH2 |
| PUL0607 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry | porphyran | Wenyingzhuangia fucanilytica strain CZ1127 | 32520542 Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22. |
2020 Jul 1 | degradation | 22 | 8 | GH2, CBM67, CBM51, GH141, PL0, GH105, GH154, GH16_11, GH16, 3.2.1.178, GH117, GH16_11, GH16, GH16_14, GH29 |
| PUL0608 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS | human milk oligosaccharide | Roseburia hominis DSM 16839 | 32620774 Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x. |
2020 Jul 3 | degradation | 9 | 2 | 3.2.1.140, GH136, 2.4.1.211, GH112 |
| PUL0609 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS | human milk oligosaccharide | Roseburia inulinivorans DSM 16841 | 32620774 Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x. |
2020 Jul 3 | degradation | 11 | 4 | GH112, 2.4.1.211, 3.2.1.63, GH95, 3.2.1.63, GH95, GH136, 3.2.1.- |
| PUL0632 | recombinant protein expression | sucrose, kestose, nystose, inulin | Roseburia inulinivorans DSM 16841 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | CBM66, GH32 |
| PUL0633 | recombinant protein expression | sucrose, kestose, nystose | Roseburia faecis M72 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0634 | recombinant protein expression | sucrose, kestose, nystose | Eubacterium rectale ATCC 33656 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 6 | 1 | GH32 |
| PUL0635 | recombinant protein expression | sucrose, kestose, nystose, fructooligosaccharide | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 7 | 1 | CBM38, GH32, CBM66 |
| PUL0636 | recombinant protein expression | sucrose, kestose, nystose, fructooligosaccharide | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 8 | 1 | GH32, CBM66 |
| PUL0637 | recombinant protein expression | sucrose, kestose, nystose | Faecalibacterium prausnitzii A2165 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0638 | recombinant protein expression | sucrose, kestose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0639 | recombinant protein expression | sucrose, kestose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 4 | 2 | GH32, GH32 |
| PUL0640 | recombinant protein expression | sucrose, kestose, nystose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | CBM66, CBM38, GH32 |
| PUL0641 | recombinant protein expression | sucrose, kestose | Anaerostipes caccae L1-92 DSM 14662 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | GH32 |
| PUL0642 | recombinant protein expression | sucrose, kestose, nystose | Roseburia intestinalis L1-82 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 11 | 6 | GH36, GH13_31, GH13, CBM38, GH32, GH13_18, GH10, CBM86, CBM9, CBM0, CBM22, GH53, CBM61 |
| PUL0646 | recombinant protein expression, crystallization, affinity gel electrophoresis, isothermal titration calorimetry | beta-glucan | Bacteroides fluxus YIT 12057 | 33587952 Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J Biol Chem. 2021 Jan-Jun;296:100415. doi: 10.1016/j.jbc.2021.100415. Epub 2021 Feb 13. |
2021 Jan-Jun | degradation | 6 | 2 | GH3, CBM6, GH158 |
| PUL0648 | high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization | glucuronoarabinoxylan | Dysgonomonas mossii DSM 22836 | 33667545 A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2. |
2021 Jan-Jun | degradation | 37 | 20 | CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67 |
| PUL0650 | enzyme activity assay, high performance anion exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay | arabinogalactan | Bifidobacterium longum JCM 7052 | 33674431 Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27. |
2021 Apr 27 | degradation | 7 | 2 | GH36, GH39, CBM35 |
| PUL0652 | RNA-Seq, enzyme activity assay, thin layer chromatography, liquid chromatography, mass spectrometry | agar | Colwellia echini A3 | 33811026 A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26. |
2021 May 26 | degradation | 54 | 10 | CBM35, GH2, CBM6, GH96, CBM6, GH96, GH50, GH50, GH50, GH29, CBM13, GH50, GH86, GH117 |
| PUL0656 | gene deletion mutant and growth assay, complementation study, clone and expression, isothermal titration calorimetry | exopolysaccharide | Bacillus cereus ATCC 10987 | 32236137 Discovery and characterization of a Gram-positive Pel polysaccharide biosynthetic gene cluster. PLoS Pathog. 2020 Apr 1;16(4):e1008281. doi: 10.1371/journal.ppat.1008281. eCollection 2020 Apr. |
2020 Apr | biosynthesis | 6 | 2 | GH0, GH166, GT4 |
| PUL0657 | recombinant protein expression, NMR | levoglucosan | Bacillus smithii S-2701M | 33208778 Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction. Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8. |
2020 Nov 18 | degradation | 8 | 2 | GH109, GH109 |
| PUL0662 | thin layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression | β-mannan | Phocaeicola dorei DSM 17855 | 34339781 BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31. |
2021 Sep 30 | degradation | 9 | 4 | GH5_7, CE7, GH26, GH130 |
| PUL0663 | thin layer chromatography, clone and expression, recombinant protein expression | arabinogalactan protein | Bacteroides plebeius DSM17135 | 34340552 Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3. |
2021 Aug 31 | degradation | 7 | 4 | GH145, GH43_24, GH43_17, GH105 |
| PUL0664 | thin layer chromatography, clone and expression, recombinant protein expression | arabinogalactan protein | Bacteroides plebeius DSM17135 | 34340552 Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3. |
2021 Aug 31 | degradation | 17 | 8 | GH49, GH2, GH36, GH27, GH28, GH43, GH43, GH2 |
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