| Basic Information | |
|---|---|
| Species | Arabidopsis lyrata |
| Cazyme ID | 341506 |
| Family | GH32 |
| Protein Properties | Length: 574 Molecular Weight: 64882.8 Isoelectric Point: 9.5448 |
| Chromosome | Chromosome/Scaffold: 3 Start: 5644536 End: 5646716 |
| Description | cell wall invertase 5 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 47 | 366 | 0 |
| HFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNETVWGHATSTDLINWITLSPAIKPSRPSDINGCWSGSVTILTNGKPVIIYTGNDRYNRQVQNLAK PKNLTDPYLRHWTKSPENPLVTPNAANHINSTAFRDPTTAWLGRDKRWRITTGSQEGRRGLAILHTSRDFVRWKQSPKPLHYHEGTGIWECPDFFPVSRT DSRGLDTSSSAGPMIKHVLKVSLTDTFRDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNIKKGWAGL QAIPREVWLDKSGKRLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 574 Download |
| MATIVWCNIA IFLLVLFFLA DNAIVVLDAL HNVPNNIKNQ PYRTGYHFQP PKNWMNGPMI 60 YKGIYHLFYQ WNQNGAVMDV NETVWGHATS TDLINWITLS PAIKPSRPSD INGCWSGSVT 120 ILTNGKPVII YTGNDRYNRQ VQNLAKPKNL TDPYLRHWTK SPENPLVTPN AANHINSTAF 180 RDPTTAWLGR DKRWRITTGS QEGRRGLAIL HTSRDFVRWK QSPKPLHYHE GTGIWECPDF 240 FPVSRTDSRG LDTSSSAGPM IKHVLKVSLT DTFRDYYTIG TYDEVRDVYV PDKGFVQDET 300 APRYDYGKFY ASKTFYDSVN QRRILWGWVN ESSPEKDNIK KGWAGLQAIP REVWLDKSGK 360 RLVQWPVKEI ERLRTTQVKW VNKVLKGGGS VIEVHGVTAS QADVEVFFKV SGLDLEKADV 420 IEPGWTDPQL ICSEKNASFV NSGLGPFGLM VLASKDMEEY TSVNFRIFRA RGNNKEHLVV 480 MCSDQSRSSL EKGNDKTTYG AFMDISPYQP ISLRTLIDKS IVESFGGKGK TCITSRVYPK 540 LATGERTHLF AFNKGSQNVD ILSLSAWSMK SSL* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-45 | 41 | 540 | 521 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-55 | 39 | 539 | 516 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-85 | 53 | 369 | 333 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-134 | 47 | 366 | 331 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 7.0e-168 | 47 | 529 | 496 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2QQU | 0 | 39 | 572 | 1 | 534 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D239a) From Arabidopsis Thaliana In Complex With Sucrose |
| DDBJ | BAB01930.1 | 0 | 22 | 572 | 21 | 578 | beta-fructofuranosidase (EC 3.2.1.26) [Arabidopsis thaliana] |
| RefSeq | NP_187994.1 | 0 | 1 | 573 | 1 | 569 | AtcwINV5 (Arabidopsis thaliana cell wall invertase 5); hydrolase, hydrolyzing O-glycosyl compounds |
| RefSeq | NP_566464.1 | 0 | 22 | 572 | 21 | 581 | ATBFRUCT1; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| Swiss-Prot | Q9LIB9 | 0 | 1 | 573 | 1 | 572 | INV5_ARATH RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme CWINV5; AltName: Full=Cell wall invertase 5; Short=AtcwINV5; AltName: Full=Sucrose hydrolase 5; AltName: Full=Cell wall beta-fructosidase 5; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qqu_A | 0 | 39 | 572 | 1 | 534 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D239a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2qqw_A | 0 | 39 | 572 | 1 | 534 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 39 | 572 | 1 | 534 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 39 | 572 | 1 | 534 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 39 | 572 | 1 | 534 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
