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Basic Information | |
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Species | Thellungiella halophila |
Cazyme ID | Thhalv10007016m |
Family | GH32 |
Protein Properties | Length: 661 Molecular Weight: 73396.8 Isoelectric Point: 5.8239 |
Chromosome | Chromosome/Scaffold: 5 Start: 11387613 End: 11391667 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 121 | 441 | 0 |
HFQPEQNWMNDPNGPLFYKGYYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWVHLPLAMVADQWYDSNGVWTGSATILPDGSIVMLYTGSTDKSVQVQNLA YPEDPNDPLLLKWVKFSGNPVLVPPPGILPKDFRDPTTAWKTSEGKWRITIGSKINRTGISLVYDTTDFKTYERLDTLLHAVPNTGMWECVDFYPVSKTD VNGLETSVNGPDVKHIVKASMDDTRIDHYAVGTYFDSNGTWIPDDPTIDVGISTSLRYDYGKFYASKTFYDQNKSRRILWGWISESDSEAADVQKGWSSL QGIPRTVVLDTKTGKNLVQWP |
Full Sequence |
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Protein Sequence Length: 661 Download |
MASSDALLPI SAREEDPLLS DGSRSDPNLE THFRRRPVKG LLAVSFGLLF IAFYVALIAT 60 HDGSRSKDAI IDETATSTSR ARLAGVSEKS NDQLWKLSGD RNTAAFAWNN SMLSWQRTAF 120 HFQPEQNWMN DPNGPLFYKG YYHFFYQYNP NAAVWGDIVW GHAVSKDLIH WVHLPLAMVA 180 DQWYDSNGVW TGSATILPDG SIVMLYTGST DKSVQVQNLA YPEDPNDPLL LKWVKFSGNP 240 VLVPPPGILP KDFRDPTTAW KTSEGKWRIT IGSKINRTGI SLVYDTTDFK TYERLDTLLH 300 AVPNTGMWEC VDFYPVSKTD VNGLETSVNG PDVKHIVKAS MDDTRIDHYA VGTYFDSNGT 360 WIPDDPTIDV GISTSLRYDY GKFYASKTFY DQNKSRRILW GWISESDSEA ADVQKGWSSL 420 QGIPRTVVLD TKTGKNLVQW PVEEIKSLRL SSKKFDMEVS PGSMIPIDVG SAAQLDIEAE 480 FEIKKESIDK ILGNASVVAE AEEFSCQNSG GSTVRGALGP FGFSVLADEK LSEQTPIYFY 540 VAKGKDSKLK TFFCTDTSRS SFANDVIKPI YGSVVPVLKG EKLTMRILVD HSIVEGFGQG 600 GRTCITSRVY PTKAIYGAAK LFLFNNAIDA TVTASFTVWQ MNSAFIHPYS EDVVRALSRN 660 * 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-57 | 117 | 611 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 9.0e-75 | 117 | 640 | 534 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 7.0e-97 | 127 | 444 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-153 | 121 | 441 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 121 | 601 | 491 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAG36942.1 | 0 | 1 | 658 | 1 | 661 | AF274298_1 acid invertase [Brassica oleracea] |
GenBank | AAG36943.1 | 0 | 1 | 659 | 1 | 661 | AF274299_1 acid invertase [Brassica oleracea] |
EMBL | CAA66330.1 | 0 | 5 | 659 | 1 | 659 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_563901.1 | 0 | 1 | 659 | 1 | 663 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 652 | 1 | 640 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 102 | 649 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugh_A | 0 | 102 | 649 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugg_B | 0 | 102 | 649 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugg_A | 0 | 102 | 649 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugf_B | 0 | 102 | 649 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |