| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si021569m |
| Family | GH32 |
| Protein Properties | Length: 589 Molecular Weight: 65063 Isoelectric Point: 5.612 |
| Chromosome | Chromosome/Scaffold: 3 Start: 1768540 End: 1771391 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 42 | 364 | 0 |
| HFQPAKNWQNDPNGPMYYNGMYHFFYQYNPHGALWDIGNLSWGHSVSGDLVNWAALDTAIDPTAPFDINGCWSGSATILRGGTPAILYTGIDANKEQVQN LAFPKNPADPLLREWHKPSYNPVIPLPADVPGDKFRDPSTAWLGRDGLWRIAVSAEVSGVASTLVYRSADFVHWERNDAPLHSSRAAGMVECPDLFPVKA HGEEGGLDTSASGAGVSHVLKLSVMDTLQDYYMVGRYDDAADAFVPAEPERGDDVRNWRRFDYGHVYAAKSFFDARRNRRVLWAWANESDSQADDVARGW SGVQTVPRKLWLDKDGKQLRQWP | |||
| Full Sequence |
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| Protein Sequence Length: 589 Download |
| MAGLPLSAVA FYLCLLASSS SALRVAPATT SEGRHVRRTA YHFQPAKNWQ NDPNGPMYYN 60 GMYHFFYQYN PHGALWDIGN LSWGHSVSGD LVNWAALDTA IDPTAPFDIN GCWSGSATIL 120 RGGTPAILYT GIDANKEQVQ NLAFPKNPAD PLLREWHKPS YNPVIPLPAD VPGDKFRDPS 180 TAWLGRDGLW RIAVSAEVSG VASTLVYRSA DFVHWERNDA PLHSSRAAGM VECPDLFPVK 240 AHGEEGGLDT SASGAGVSHV LKLSVMDTLQ DYYMVGRYDD AADAFVPAEP ERGDDVRNWR 300 RFDYGHVYAA KSFFDARRNR RVLWAWANES DSQADDVARG WSGVQTVPRK LWLDKDGKQL 360 RQWPIEEIET LRRKRVGLRR NTMLSAGAMN EIVGIAEAQA DVEVAFRIPS LEEAEALDSN 420 WLLDPQKLCG EKGASVPGGV GPFGLIVMAS GDLQEHTAVF FRVFRHDDKY KVLMCTDLTR 480 SSARAGVYKP PYGGFVDIDI EERKTIKLRT LVDHSVVESY GAEGRACITA RVYPEHVETS 540 NSHMFVFNNG TDKVKVTKLE AWELAAATVN VGDEGLIVSE SKDESESY* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 0.0002 | 478 | 558 | 81 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| COG1621 | SacC | 1.0e-60 | 38 | 373 | 357 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-88 | 48 | 367 | 332 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-134 | 42 | 364 | 331 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 9.0e-166 | 42 | 524 | 493 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| Swiss-Prot | A2YZ01 | 0 | 3 | 571 | 5 | 574 | INV7_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7; AltName: Full=Sucrose hydrolase 7; AltName: Full=Invertase 7; AltName: Full=Cell wall beta-fructosidase 7; AltName: Full=OsCIN7; Flags: Precursor |
| GenBank | AAD38399.1 | 0 | 3 | 588 | 5 | 594 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
| GenBank | EEC84182.1 | 0 | 3 | 571 | 5 | 573 | hypothetical protein OsI_30564 [Oryza sativa Indica Group] |
| RefSeq | NP_001062691.1 | 0 | 3 | 571 | 5 | 574 | Os09g0255000 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002448713.1 | 0 | 1 | 588 | 1 | 599 | hypothetical protein SORBIDRAFT_06g031930 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 38 | 571 | 8 | 535 | A Chain A, The Xylanase Ta From Thermoascus Aurantiacus Utilizes Arabinose Decorations Of Xylan As Significant Substrate Specificity Determinants |
| PDB | 2ade_A | 0 | 38 | 571 | 8 | 535 | A Chain A, The Xylanase Ta From Thermoascus Aurantiacus Utilizes Arabinose Decorations Of Xylan As Significant Substrate Specificity Determinants |
| PDB | 2add_A | 0 | 38 | 571 | 8 | 535 | A Chain A, The Xylanase Ta From Thermoascus Aurantiacus Utilizes Arabinose Decorations Of Xylan As Significant Substrate Specificity Determinants |
| PDB | 1st8_A | 0 | 38 | 571 | 8 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 38 | 571 | 8 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
