Basic Information | |
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Species | Phaseolus vulgaris |
Cazyme ID | Phvul.011G005900.1 |
Family | GH32 |
Protein Properties | Length: 628 Molecular Weight: 70781.5 Isoelectric Point: 6.5175 |
Chromosome | Chromosome/Scaffold: 11 Start: 439550 End: 444057 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 112 | 430 | 0 |
HFQPQKNWMNDPNGPLFYKGWYHIFYQYNPESAVFGNITWGHAVSTDLIHWLYLPIAMLRDSWYDAKGVWSGSATLLPDGRIVMLYTGNTHQNVQVQNLA YPANLSDPLLLHWVKYADNPVVLPPPGIGPKDFRDPTTAWVGPDDKWRVLIGSKVNQTGLSLVYKTQDFIHYELNDEYMHEVPGTGMWECVDFYPVSVNG PDGLDTSVNGPGVKHVLKSSLDDTRGDHYAIGRYFIENDTWVPDDPNKDVGIGLKLDYGRFYASKTFYDQNKKRRVLWGWINESDSETADLKKGWASLQS IPRTVVFDKKTETHLLQWP |
Full Sequence |
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Protein Sequence Length: 628 Download |
MKKTKTKTMN PDSQIPLLIP PTSPTMESGG RPQKGTLLFI VSVLFLMPLA ALFISLHIHN 60 LQKAFRREEN SLPLFPRGVA EGVSAKSNPY LWQKGSYNWT NAMLSWQRTA FHFQPQKNWM 120 NDPNGPLFYK GWYHIFYQYN PESAVFGNIT WGHAVSTDLI HWLYLPIAML RDSWYDAKGV 180 WSGSATLLPD GRIVMLYTGN THQNVQVQNL AYPANLSDPL LLHWVKYADN PVVLPPPGIG 240 PKDFRDPTTA WVGPDDKWRV LIGSKVNQTG LSLVYKTQDF IHYELNDEYM HEVPGTGMWE 300 CVDFYPVSVN GPDGLDTSVN GPGVKHVLKS SLDDTRGDHY AIGRYFIEND TWVPDDPNKD 360 VGIGLKLDYG RFYASKTFYD QNKKRRVLWG WINESDSETA DLKKGWASLQ SIPRTVVFDK 420 KTETHLLQWP VEEIESLRLR SYEFEEVVVK PGSVVPLDIG PATQLDIFAE FEIEYLASKG 480 TGKKENVGCG NGAFDRSSLG PFGILAIADD KLSELTPIYF HLSNTSFFCV DETRSSKASD 540 VSKLVFGSEV PVVGDERLSM RVLVDHSIIE SFAQGGRTVI SSRVYPTEAI YEAARLFVFN 600 NATDINIKAS LKIWQLNSAF IRPFPFH* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 7.0e-52 | 108 | 586 | 496 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 7.0e-73 | 108 | 618 | 526 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 6.0e-96 | 118 | 433 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-149 | 112 | 430 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 112 | 576 | 475 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 12 | 624 | 2 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF34363.1 | 0 | 76 | 626 | 84 | 639 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35858.1 | 0 | 57 | 626 | 68 | 642 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 57 | 626 | 68 | 642 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 76 | 626 | 84 | 636 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 97 | 624 | 8 | 544 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
PDB | 3ugh_A | 0 | 97 | 624 | 8 | 544 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
PDB | 3ugg_B | 0 | 97 | 624 | 8 | 544 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
PDB | 3ugg_A | 0 | 97 | 624 | 8 | 544 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
PDB | 3ugf_B | 0 | 97 | 624 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |