Basic Information | |
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Species | Vitis vinifera |
Cazyme ID | GSVIVT01007789001 |
Family | GT20 |
Protein Properties | Length: 718 Molecular Weight: 80758.6 Isoelectric Point: 6.3331 |
Chromosome | Chromosome/Scaffold: 17 Start: 8992843 End: 8998935 |
Description | trehalose-phosphatase/synthase 9 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 143 | 417 | 0 |
RFYRVKLGFFLHSPFPSSEIYRTLPVRDDILKALLNADLVGFHTFDYARHFLSCCSRMLGLNYESKRGHIGLEYFGRTVYVKILPVGIHMGQLESALNLP STSIKVKEIQEQFKGKKIILGVDDMDIFKGLSLKLLAMEHLLQHYEELRGELVLVQIVNPARSTGKDVQEAKRETYAITERINANFGFPGYEPVVLIDHP VPFYEKTAYYALAECCIVNAVRDGMNLMPYNYIVCRQGTPKIDEALGITSGSSRTSTLVVSEFIGCSPSLSGAIR |
Full Sequence |
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Protein Sequence Length: 718 Download |
MVSRSSYTSF LDAASGDLLN FPQTPRTLPR VMTVPGIISD VANFLPLLAQ KDLNTGRWCF 60 SFDEDALLLQ MKDGFSSETD VVYVGSLKVD VDTSEQEEVA ERLLAEFNCV PTFLPPDLQK 120 KFYHGFCKQY LWPLFHYMLP IKRFYRVKLG FFLHSPFPSS EIYRTLPVRD DILKALLNAD 180 LVGFHTFDYA RHFLSCCSRM LGLNYESKRG HIGLEYFGRT VYVKILPVGI HMGQLESALN 240 LPSTSIKVKE IQEQFKGKKI ILGVDDMDIF KGLSLKLLAM EHLLQHYEEL RGELVLVQIV 300 NPARSTGKDV QEAKRETYAI TERINANFGF PGYEPVVLID HPVPFYEKTA YYALAECCIV 360 NAVRDGMNLM PYNYIVCRQG TPKIDEALGI TSGSSRTSTL VVSEFIGCSP SLSGAIRHYR 420 FRIVALSPNF RKLSLDHIVK AYKRANRRAI FLDYDGTVVP QSSIVKTPSP EVISILNDLC 480 NDPKNTVFIV SGRGKNSLSD WFAQCQNLGI AAEHGYFIRW SQSSNWESRP LLMDFDWKRI 540 ADPVMQLYTE ATDGSYIETK ESALVWHHQD ADPDFGSCQA MELLDHLENV LANEPVEVKR 600 GHHIVEVKPQ GVSKGQVTEK ILSTMISDGK PPDFVMCIGD DRSDEDMFES ISSTIYSPSL 660 PAPPEIFACT VGQKPSKARY YLDDSADVLK LLQGLARASS MKPKCSTQIQ FSFESVA* 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam02358 | Trehalose_PPase | 2.0e-96 | 451 | 686 | 239 | + Trehalose-phosphatase. This family consist of trehalose-phosphatases EC:3.1.3.12 these enzyme catalyze the de-phosphorylation of trehalose-6-phosphate to trehalose and orthophosphate. The aligned region is present in trehalose-phosphatases and comprises the entire length of the protein it is also found in the C-terminus of trehalose-6-phosphate synthase EC:2.4.1.15 adjacent to the trehalose-6-phosphate synthase domain - pfam00982. It would appear that the two equivalent genes in the E. coli otsBA operon otsA the trehalose-6-phosphate synthase and otsB trehalose-phosphatase (this family) have undergone gene fusion in most eukaryotes. Trehalose is a common disaccharide of bacteria, fungi and invertebrates that appears to play a major role in desiccation tolerance. | ||
cd03788 | GT1_TPS | 3.0e-131 | 41 | 417 | 424 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
PLN03063 | PLN03063 | 1.0e-132 | 41 | 700 | 805 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
pfam00982 | Glyco_transf_20 | 3.0e-160 | 41 | 417 | 431 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
PLN02205 | PLN02205 | 0 | 1 | 699 | 849 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAN78769.1 | 0 | 1 | 715 | 1 | 855 | hypothetical protein [Vitis vinifera] |
EMBL | CBI15059.1 | 0 | 1 | 717 | 1 | 717 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002283215.1 | 0 | 1 | 717 | 1 | 862 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002318643.1 | 0 | 1 | 716 | 1 | 856 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002511428.1 | 0 | 1 | 717 | 1 | 861 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1gz5_D | 2e-28 | 131 | 416 | 126 | 398 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_C | 2e-28 | 131 | 416 | 126 | 398 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_B | 2e-28 | 131 | 416 | 126 | 398 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_A | 2e-28 | 131 | 416 | 126 | 398 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1uqu_B | 2e-28 | 131 | 416 | 127 | 399 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 771 | 54 | 699 | 0 |
HO779327 | 522 | 69 | 469 | 0 |
HO779327 | 253 | 466 | 718 | 0 |
DT735322 | 297 | 421 | 717 | 0 |
HO797286 | 468 | 301 | 699 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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